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Journal
2012 | 10 | 4 | 1073-1078
Article title

Impact of un-polymerized acrylamide monomer residues onto protein identification by MALDI TOF MS

Content
Title variants
Languages of publication
EN
Abstracts
EN
Polyacrylamide gel electrophoresis is a powerful tool for protein mixture separation frequently used in proteomic studies. This article describes the problems which can arise during the identification process of separated proteins by matrix assisted laser desorption/ionization mass spectrometry. Presence of residual acrylamide monomers in the peptide samples leads to a significant peptide signal suppression. In the case of protein prohibitin, isolated from the MCF 7 breast cancer cell line, the peptide signal suppression was observed in 50% of all detected peptides. [...]
Publisher

Journal
Year
Volume
10
Issue
4
Pages
1073-1078
Physical description
Dates
published
1 - 8 - 2012
online
29 - 5 - 2012
Contributors
author
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia, ivan.talian@upjs.sk
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
author
  • Department of Medical and Clinical Biophysics, Pavol Jozef Šafárik University, 040 11, Košice, Slovakia
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-012-0024-3
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