PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2012 | 10 | 2 | 407-412
Article title

Using 2-D electrophoresis followed by nano HPLC in nuclear protein analysis of MCF-7 breast cancer cell line by MALDI-TOF/TOF

Content
Title variants
Languages of publication
EN
Abstracts
EN
The biomarker identification is an important tool in early cancer detection. The MCF-7 breast cancer cell line was chosen as a model system. The nuclear proteins were extracted utilizing a commercially manufactured kit and separated on two dimensional (2-D) sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). The first dimension was performed on isoelectric focusing strips with pH range 4–7. Afterwards the proteins were tryptic digested and identification was performed by matrix assisted laser desorption technique with time of flight mass analyzer (MALDI-TOF/TOF). For unambiguous identification proteins with too low concentration or spots contains protein mixture the nano high performance liquid chromatography (HPLC) was used. The 2-D gel electrophoresis (2-DGE) seems to be a good tool to separate large amount of proteins using relatively simple procedure and its hyphenation with HPLC can create the perfect analytical solution for proteome identification. About 150 nuclei protein spots were visualized and the most abundant of them were identified. [...]
Publisher

Journal
Year
Volume
10
Issue
2
Pages
407-412
Physical description
Dates
published
1 - 4 - 2012
online
29 - 1 - 2012
Contributors
  • Department of Medical Biophysics, Faculty of Medicine, University of Pavol Jozef Šafárik, 040 11, Košice, Slovakia
author
  • Department of Medical Biophysics, Faculty of Medicine, University of Pavol Jozef Šafárik, 040 11, Košice, Slovakia, ivan.talian@upjs.sk
  • Department of Medical Biophysics, Faculty of Medicine, University of Pavol Jozef Šafárik, 040 11, Košice, Slovakia
author
  • Department of Medical Biophysics, Faculty of Medicine, University of Pavol Jozef Šafárik, 040 11, Košice, Slovakia
References
  • [1] X. Zhang, A. Fang, C.P. Riley, M. Wang, F.E. Regnier, Ch. Buck, Analytica Chimica Acta 664, 101 (2010) http://dx.doi.org/10.1016/j.aca.2010.02.001[Crossref]
  • [2] R. Pieper, C.L. Gatlin, A.J. Makusky, P.S. Russo, C.R. Schatz, S.S. Miller, Q. Su, A.M. McGrath, M.A. Estock, P.P. Parmar, M. Zhao, S. Huang, J. Zhou, F. Wang, R. Esquer-Blasco, N.L. Anderson, J. Taylor, S. Steiner, Proteomics 3, 1345 (2003) http://dx.doi.org/10.1002/pmic.200300449[Crossref]
  • [3] R.I. Somiari, A. Sullivan, S. Russell, S. Somiari, H. Hu, R. Jordan, A. George, R. Katenhusen, A. Buchowiecka, C. Arciero, H. Brzeski, J. Hooke, C. Shriver, Proteomics 3, 1863 (2003) http://dx.doi.org/10.1002/pmic.200300560[Crossref]
  • [4] C. Pionneau, L. Canelle, J. Bousquet, J. Hardouin, J. Bigeard, M. Caron, R. Joubert-Caron, Cancer Genomics Proteomics 2, 199 (2005)
  • [5] R.J. Simpson, L.M. Connoly, J.S. Eddes, J.J. Pereira, R.L. Moritz, G.E. Reid, Electrophoresis 21, 1707 (2000) http://dx.doi.org/10.1002/(SICI)1522-2683(20000501)21:9<1707::AID-ELPS1707>3.0.CO;2-Q[Crossref]
  • [6] F. Poirier, R. Joubert-Caron, V. Labas, M. Caron, Proteomics 3, 1028 (2003) http://dx.doi.org/10.1002/pmic.200300374[Crossref]
  • [7] P.J. Adam, R. Boyd, K.K. Tyson, G.C. Fletcher, A. Stamps, L. Hudson, H.R. Poyser, N. Redpath, M. Griffiths, G. Steers, A.L. Harris, S. Patel, J. Berry, J.A. Loader, R.R. Townsend, L. Daviet, P. Legrain, R. Parekh, J.A. Terrett, J. Biol. Chem. 278, 6482 (2003) http://dx.doi.org/10.1074/jbc.M210184200[Crossref]
  • [8] L. Malorni, G. Cacace, M. Cuccurullo, G. Pocsfalvi, A. Chambery, A. Farina, A. Di Maro, A. Parente, A. Malorni, Proteomics 6, 5973 (2006) http://dx.doi.org/10.1002/pmic.200600333[Crossref]
  • [9] Y. Ruan, W. Pei, M. Wan, Cell Biochem. Funct. 26, 787 (2008) http://dx.doi.org/10.1002/cbf.1507[Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-011-0156-x
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.