Optimization study of the catalytic activity of DNAzymes based on telomeric G-quadruplexes

• Authors: Joanna Kosman , Bernard Juskowiak
• Languages of publication: EN

Abstracts

EN

Optimization studies of the procedure for peroxidase activity measurements with DNAzymes based on telomeric sequences and colorimetric indicator reactions are reported. Effect of metal cation, nature and concentration of surfactant, as well as thermal treatment of G-quadruplex sample are investigated. Nature of metal cation exhibited modest influence on the system performance. Great improvement of enzymatic activity of the telomeric quadruplexes in the presence of Brij 58 surfactant was observed. Further improvement of catalytic activity of the system based on human telomeric sequence was attained by applying a thermal treatment (heating/rapid cooling) procedure to prepare G-quadruplex/hemin complexes. [...]

Keywords

EN

DNAzyme; G-quadruplex; Peroxidase activity; Brij58; Telomerase assay

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2012
• Volume: 10
• Issue: 2
• Pages: 368-372

Dates

published

1 - 4 - 2012

online

29 - 1 - 2012

Contributors

author

Joanna Kosman

• Faculty of Chemistry, Adam Mickiewicz University, 60-780, Poznan, Poland

author

Bernard Juskowiak

• Faculty of Chemistry, Adam Mickiewicz University, 60-780, Poznan, Poland

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Identifiers

DOI

10.2478/s11532-011-0152-1