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2011 | 9 | 5 | 886-895
Article title

Osmium assay in fixatives and stained rat tissues by means of acid and o,o′-dihydroxo substituted monoazo dyes and some flavonoids

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EN
Abstracts
EN
New simple, rapid and accurate spectrophotometric techniques for osmium assays in fixatives and stained tissues have been elaborated using acid (Tropaeolin O, Tropaeolin OOO-I, Tropaeolin OOO-II, Orange G), o,o′-dihydroxo substituted azo dyes (Eriochrome Blue Black R, Acid Chrome Dark-Blue, Eriochrome Black T) and flavonoids (morin, quercetin, luteolin). Methods are based on sensitive osmium(IV) reactions with reagents and the formation of stable coloured compounds. Tolerance ratios of main matrix components of histological specimens during Os(IV) spectrophotometric determination with reagents has been established. Results of osmium determination in fixatives and fixed tissues, obtained by means of different reagents, appeared to be sufficiently similar, although effects of matrix components differ. The accuracy of spectrophotometric osmium assay in fixatives with azo dyes has been confirmed with voltammetric investigations. Results obtained from osmium uptake by rat gum tissues are valuable for clinical testing of dental drugs, indicating the influence of drug treatment on the gums. [...]
Publisher

Journal
Year
Volume
9
Issue
5
Pages
886-895
Physical description
Dates
published
1 - 10 - 2011
online
24 - 7 - 2011
Contributors
  • Analytical Chemistry Department, Chemistry Faculty, Ivan Franko Lviv National University, 79005, Lviv, Ukraine, marianne_polko@yahoo.com
  • Analytical Chemistry Department, Chemistry Faculty, Ivan Franko Lviv National University, 79005, Lviv, Ukraine
  • Analytical Chemistry Department, Chemistry Faculty, Ivan Franko Lviv National University, 79005, Lviv, Ukraine
author
  • Analytical Chemistry Department, Chemistry Faculty, Ivan Franko Lviv National University, 79005, Lviv, Ukraine
  • Analytical Chemistry Department, Chemistry Faculty, Ivan Franko Lviv National University, 79005, Lviv, Ukraine
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-011-0070-2
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