The computational modelling of the kinetics of ascorbic acid palmitate hydrolysis by lipase considering diffusion

• Authors: Jurgita Dabulytė-Bagdonavičienė , Feliksas Ivanauskas , Valdemaras Razumas
• Languages of publication: EN

Abstracts

EN

This paper presents mathematical and computational modelling of kinetics of a bioelectroanalytical system based on the interfacial action of hydrolytic enzyme. A system of non-linear differential equations with diffusion is used to describe the kinetics of Termomyces lanuginosa lipase (TLL) catalyzed hydrolysis of L-ascorbic acid palmitate (AAP). The system was solved numerically, and the kinetic prameters of AAP hydrolysis by the enzyme were determined. The experimental and modelling results show linear dependence of the rate of AAP hydrolysis on the TLL concentration. Complex dependence of the initial rate of bioelectrocatalytic current increase on the thickness of total diffusion layer (hydrodynamic diffusion layer plus thickness of dialysis membrane on the electrode surface) is also demonstrated and explained. [...]

Keywords

EN

Ascorbic acid; Lipase; Enzyme reaction; Mathematical model; Computational model

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2011
• Volume: 9
• Issue: 4
• Pages: 712-719

Dates

published

1 - 8 - 2011

online

4 - 6 - 2011

Contributors

author

Jurgita Dabulytė-Bagdonavičienė

author

Feliksas Ivanauskas

• Department of Computer Science, Vilnius University, LT-03225, Vilnius, Lithuania

author

Valdemaras Razumas

• Institute of Biochemistry, Vilnius University, LT-08662, Vilnius, Lithuania

References

• [1] S.R. Pinnell, H. Yang, M. Omar, N.M. Riviere, H.V. DeBuys, L.C. Walker, Y. Wang, M. Levine, Dermatol. Surg. 27, 137 (2001) http://dx.doi.org/10.1046/j.1524-4725.2001.00264.x[Crossref]
• [2] Z. Tao, Y. Ren, W. Tong, D. Wei, Pharm. Rep. 57, 77 (2005)
• [3] D.L. Nelson, M.M. Cox, Leninger Principles of Biochemistry (Freeman, New York 2005)
• [4] M. Pokorski, M. Marczak, A. Dymecka, P. Suchocki, J. Biomed. Sci. 10, 193 (2003) http://dx.doi.org/10.1007/BF02256054[Crossref]
• [5] E. Sottofattori, M. Anazaldi, A. Balbi, G. Tonelio, J. Pharm. Biomed. Anal. 18, 213 (1998) http://dx.doi.org/10.1016/S0731-7085(98)00173-3[Crossref]
• [6] C.C. Wang, A.M. Wu, Anal. Chim. Acta, 576, 124 (2006) http://dx.doi.org/10.1016/j.aca.2005.12.017[Crossref]
• [7] B. Kazakevičienė, PhD thesis, Institute of Biochemistry (Publishing House of Vilnius University, Vilnius, 2006) (in Lithuanian)
• [8] B. Kazakevičienė, G. Valinčius, G. Niaura, Z. Talaikytė, M. Kažemėkaitė, V. Razumas, J. Phys. Chem. B 107, 6661 (2003) http://dx.doi.org/10.1021/jp035048o[Crossref]
• [9] G. Valinčius, G. Niaura, B. Kazakevičienė, Z. Talaikytė, M. Kažemėkaitė, E. Butkus, V. Razumas, Langmuir 20, 6631 (2004) http://dx.doi.org/10.1021/la0364800[Crossref]
• [10] B. Kazakevičienė, G. Valinčius, G. Niaura, Z. Talaikytė, M. Kažemėkaitė, V. Razumas, D. Plaušinaitis, A. Teišerskienė, V. Lisauskas, Langmuir 23, 4965 (2007) http://dx.doi.org/10.1021/la0632169[Crossref]
• [11] A. Houde, A. Kademi, D. Leblanc, Appl. Biochem. Biotech. 118, 155 (2004) http://dx.doi.org/10.1385/ABAB:118:1-3:155[Crossref]
• [12] G. Valinčius, I. Ignatjev, G. Niaura, M. Kažemėkaitė, Z. Talaikytė, V. Razumas, A. Svendsen, Anal. Chem. 77, 2632 (2005) http://dx.doi.org/10.1021/ac048230+[Crossref]
• [13] I. Ignatjev, G. Valinčius, I. Švedaitė, E. Gaidamauskas. M. Kažemėkaitė, V. Razumas, A. Svendsen, Anal. Biochem. 344, 275 (2005) http://dx.doi.org/10.1016/j.ab.2005.06.014[Crossref]
• [14] M. Puida F. Ivanauskas, I. Ignatjev, G. Valinčius, V. Razumas, Nonlinear analysis: modelling and control 12(2), 245 (2007)
• [15] R. Verger, M.C.E. Mieras, G.H. De Haas, J. Biol. Chem. 248, 4023 (1973)
• [16] K. Kumbhakart, T. Goel, T. Mukherjee, H. Pal, J. Phys. Chem. B 108, 19246 (2004) http://dx.doi.org/10.1021/jp0468004[Crossref]
• [17] P. Becher, J. Phys. Chem. 66, 374 (1962) http://dx.doi.org/10.1021/j100808a517[Crossref]
• [18] P. Manimozhi, L. Rajendran, J. Electroanal. Chem. 647, 87 (2010) http://dx.doi.org/10.1016/j.jelechem.2010.05.019[Crossref]
• [19] R. Senthamarai, L. Rajendran, Electrochim. Acta. 55, 3223 (2010) http://dx.doi.org/10.1016/j.electacta.2010.01.013[Crossref]
• [20] D.A. Gough, J.K Leypoldt, Anal. Chem. 51, 439 (1976) http://dx.doi.org/10.1021/ac50039a028[Crossref]
• [21] A.A. Samarskii, The Theory of Difference Schemes (Marcel Dekker, New York - Basel, 2001) http://dx.doi.org/10.1201/9780203908518[Crossref]
• [22] R. Baronas, F. Ivanauskas, J. Kulys, et al., J. Math. Chem. 34, 227 (2003) http://dx.doi.org/10.1023/B:JOMC.0000004072.97338.12[Crossref]
• [23] R. Baronas, J. Kulys, F. Ivanauskas, Biosens. Bioelectron. 19, 915 (2004) http://dx.doi.org/10.1016/j.bios.2003.08.022[Crossref]
• [24] R. Baronas, J. Kulys, F. Ivanauskas, J. Math. Chem. 39, 345 (2006) http://dx.doi.org/10.1007/s10910-005-9034-0[Crossref]
• [25] R. Baronas, F. Ivanauskas, J. Kulys, Mathematical Modeling of Biosensors. An Introduction for Chemists and Mathematicians (Springer, Springer Series on Chemical Sensors and Biosensors, Dordrecht, 2010) [WoS]
• [26] J.B. Raoof, R. Ojani, R. Hosseinzaden, V. Dhasemi, Anal. Sci. 19, 1251 (2003) http://dx.doi.org/10.2116/analsci.19.1251[Crossref]
• [27] A.J. Bard, L.R. Faulkner, Electrochemical Methods: Fundamentals and Applications (Wiley, New York, 2001)

Identifiers

DOI

10.2478/s11532-011-0053-3