The feasibility of the application of various eluents for recovery of lead and cadmium from Saccharomyces biomass has been investigated. Desorbing agents such as HCl, HNO3, H2SO4, Na2SO4, Na2CO3, EDTA and NaOH at concentrations of 0.05–0.5 M were used. The possibility of re-using of S. pastorianus for cadmium removal was assessed. Among the desorbing agents EDTA and mineral acids were the most effective, as approximately 85% of lead and cadmium could be recovered. However, when HNO3 and EDTA were used as desorbing agents, a dramatic loss in the cadmium adsorption by the biomass in the next cycles was observed.