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Journal
2010 | 8 | 5 | 1034-1040
Article title

Screen-printed electrodes modified with HRP-zirconium alcoxide film for the development of a biosensor for acetaminophen detection

Content
Title variants
Languages of publication
EN
Abstracts
EN
The development of a biosensor based on the immobilization of horseradish peroxidase (HRP) within a zirconium alkoxide-polyetilenimine film onto screen-printed electrodes (SPE) for acetaminophen detection and acetaminophen quantification in pharmaceutical products is described. The biosensor operation mode is based on monitoring the amperometric signal produced by the electrochemical reduction of the enzymatically generated electroactive oxidized species of acetaminophen in the presence of hydrogen peroxide. The enzyme immobilization is performed by retention in a polyethylenimine-zirconium alcoxide porous gel film, a technique that offers good entrapping and a protective environment for the biocomponent due to the hydration properties of the immobilization layer. SPEs have the advantage of being easily mass-produced at low costs with superior characteristics in comparison with classical electrode materials. In this configuration, zirconium alkoxide demonstrates its electrocatalytic activity. The biosensor allows the quantification of acetaminophen with a limit of detection of 6.21×10−8 M and a linear range between 4.35×10−7 M and 4.98×10−6 M. Finally, the biosensor is applied to the quantitative analysis of acetaminophen in Perdolan® tablets. [...]
Publisher

Journal
Year
Volume
8
Issue
5
Pages
1034-1040
Physical description
Dates
published
1 - 10 - 2010
online
5 - 9 - 2010
Contributors
author
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
author
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania
  • Analytical Chemistry Department, Faculty of Pharmacy, “IULIU HAŢIEGANU” University of Medicine and Pharmacy, Cluj-Napoca, 400349, Romania, rsandulescu@umfcluj.ro
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-010-0070-7
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