Full-text resources of PSJD and other databases are now available in the new Library of Science.
Visit https://bibliotekanauki.pl


Preferences help
enabled [disable] Abstract
Number of results


2010 | 8 | 3 | 626-632

Article title

Spectrophotometric study of time stability and acid-base characteristics of chelerythrine and dihydrochelerythrine


Title variants

Languages of publication



Time stability, acid-base and UV-VIS spectral properties of dihydrochelerythrine (DHCHE) were studied spectrophotometrically in water:methanol and water:ethanol media. DHCHE is stable in strongly acid milieu (pH HCl, HNO3, H2SO4, H3PO4 and their mixtures. Remarkable shifts of formation parts of absorbance-pH (A-pH) curves to the alkaline medium were observed depending on the type and concentration of inert electrolyte (most remarkable for HNO3 and HCl). The corresponding equilibrium constants pKR+ of the transition reaction between charged iminium Q+ and uncharged QOH (pseudo-base, 6-hydroxy-dihydro derivative) forms of chelerythine were calculated using a numerical interpretation of A-pH curves by a SQUAD-G computer program which ranged from 8.51–9.31. The highest changes of ΔpK R+ (0.75 and 0.53) were observed for H3PO4 and H2SO4, respectively. The priority effect of ionic species and ionic strength was confirmed in the presence of additions of NaCl and KCl. The strength of interaction of CHE with biomacromolecular compounds (i.e., peptides, proteins, nucleic acids etc.) may be affected because of the observed influence of both cation and anion of the inert electrolyte on acid-base behavior. [...]










Physical description


1 - 6 - 2010
25 - 4 - 2010


  • Department of Chemistry and Biochemistry, Mendel University, 613 00, Brno, Czech Republic
  • Department of Chemistry, Masaryk University, 611 37, Brno, Czech Republic
  • Department of Chemistry and Biochemistry, Mendel University, 613 00, Brno, Czech Republic
  • Department of Medical Chemistry and Biochemistry, Palacky University, 775 15, Olomouc, Czech Republic


  • [1] V. Šimánek, in: A. Brossi (Ed.), The Alkaloids (Academic Press, Orlando, 1985) 26, 185
  • [2] J. Psotová et al., J. Chromatogr. B., Analyt. Technol. Biomed. Life Sci. 830, 427 (2006)
  • [3] M. Maiti, G.S. Kumar, Med. Res. Rev. 27, 649 (2007) http://dx.doi.org/10.1002/med.20087[Crossref]
  • [4] R. Sinha, M. Hossain, G.S. Kumar, DNA Cell Biol. 28, 209 (2009) http://dx.doi.org/10.1089/dna.2008.0838[Crossref]
  • [5] J. Urbanová, P. Lubal, I. Slaninová, E. Táborská, P. Táborský, Anal. Bioanal. Chem. 394, 997 (2009) http://dx.doi.org/10.1007/s00216-009-2601-7[Crossref]
  • [6] I. Slaninová, J. Slanina, E. Táborská, Chem. Listy 102, 427 (2008) (in Czech)
  • [7] Z. Dvořák et al., Heterocycles 68, 2403 (2006) http://dx.doi.org/10.3987/REV-06-610[Crossref]
  • [8] M. Janovská, M. Kubala, V. Šimánek, J. Ulrichová, Anal. Bioanal. Chem. 395, 235 (2009) http://dx.doi.org/10.1007/s00216-009-2903-9[Crossref]
  • [9] J. Dostál, J. Slavík, In: Atta-ur-Rahman (Ed.) Studies in Natural Products Chemistry, (Elsevier, Amsterdam, 2002) 27, 155
  • [10] M. Vlčková, J. Barták, V. Kubáň, J. Chromatogr. A 1040, 141 (2004) http://dx.doi.org/10.1016/j.chroma.2004.03.064[Crossref]
  • [11] M. Vlčková, V. Kubáň, J. Vičar, V. Šimánek, Electrophoresis 26, 1673 (2005) http://dx.doi.org/10.1002/elps.200410193[Crossref]
  • [12] R. Vespalec, M. Vlčková, H. Horáková, J. Chromatogr. A 1051, 75 (2004) http://dx.doi.org/10.1016/j.chroma.2004.05.051[Crossref]
  • [13] I.G. Motevich, N.D. Strekal, J.W. Nowicky, S.A. Maskevich, J. Appl. Spectrosc. 74, 666 (2007) http://dx.doi.org/10.1007/s10812-007-0107-7[Crossref]
  • [14] P. Giri, G.S. Kumar, Biochim. Biophys. Acta 1770, 1419 (2007)
  • [15] K. Bhadra, M. Maiti, G.S. Kumar, DNA Cell Biol. 27, 675 (2008) http://dx.doi.org/10.1089/dna.2008.0779[Crossref]
  • [16] E. Vrublová et al., J. Food Chem. Toxicol. 46, 2546 (2008) http://dx.doi.org/10.1016/j.fct.2008.04.013[Crossref]
  • [17] H. Absolínová, L. Jančář, I. Jančářová, J. Vičar, V. Kubáň, Cent. Eur. J. Chem. 7, 876 (2009) http://dx.doi.org/10.2478/s11532-009-0079-y[Crossref]

Document Type

Publication order reference


YADDA identifier

JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.