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Journal
2010 | 8 | 1 | 87-95
Article title

Comparison of pressurised fluid and ultrasonic extraction methods for analysis of plant antioxidants and their antioxidant capacity

Content
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Languages of publication
EN
Abstracts
EN
The analytical method based on the high-performance liquid chromatography coupled with UV detection (HPLC/UV) for determination of selected antioxidants (i.e., esculetin, scopoletin, 7-hydroxycoumarine, rutin, xanthotoxin, 5-methoxypsoralen and quercetin) in plant material was developed. Pressurised fluid extraction (PFE) and ultrasonic extraction (USE) methods for the isolation of these compounds from ten real plant samples were used. Both extraction methods were optimised and compared to each other. For the proposed HPLC/UV method the LOQ values (limit of quantification) in the range from 22.7 (xanthotoxin) to 97.2 ng mL−1 (rutin) were obtained. For all extracts the antioxidant capacity based on the reduction of free 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) was also determined. Results ranged from 82.04 to 94.43% of DPPH radical inhibition for PFE method and from 76.01 to 89.94% in the case of USE method. [...]
Publisher

Journal
Year
Volume
8
Issue
1
Pages
87-95
Physical description
Dates
published
1 - 2 - 2010
online
16 - 2 - 2010
Contributors
author
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
author
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic, Martin.Adam@upce.cz
author
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
author
  • Faculty of Chemical Technology, Department of Analytical Chemistry, University of Pardubice, 532 10, Pardubice, Czech Republic
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-009-0125-9
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