Interaction of serum albumin with vinyl sulfonate azo dye

• Authors: Jolanta Sereikaite , Vladas-Algirdas Bumelis
• Languages of publication: EN

Abstracts

EN

The interaction between bovine serum albumin and the mono azo reactive dye Orange ZT has been investigated using absorption difference spectroscopy. The influence of pH and ionic strength of the solution on the stability of the dye-protein complex has been determined. At 25°C, the complex dissociation constants were equal to 24.0, 28.0, 7.0, 11.0, 17.6 and 46.0 μM at pH 7.0, 6.5, 6.0, 5.5, 5.0 and 4.3, respectively. In the presence of 0.1, 0.2, 0.3 M KCl, at pH 6.0 and 25°C, the complex dissociation constants were 8.8, 20.0, 18.0 μM, respectively. The protein-dye complex dissociation constants show that Orange ZT could be used as an affinity ligand for protein purification. [...]

Keywords

EN

Orange ZT; Serum albumin; Absorption difference spectroscopy; Affinity ligand

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2008
• Volume: 6
• Issue: 4
• Pages: 509-512

Dates

published

1 - 12 - 2008

online

28 - 10 - 2008

Contributors

author

Jolanta Sereikaite

• Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania

author

Vladas-Algirdas Bumelis

• Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania

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Identifiers

DOI

10.2478/s11532-008-0066-8