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Journal
2008 | 6 | 4 | 509-512
Article title

Interaction of serum albumin with vinyl sulfonate azo dye

Content
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EN
Abstracts
EN
The interaction between bovine serum albumin and the mono azo reactive dye Orange ZT has been investigated using absorption difference spectroscopy. The influence of pH and ionic strength of the solution on the stability of the dye-protein complex has been determined. At 25°C, the complex dissociation constants were equal to 24.0, 28.0, 7.0, 11.0, 17.6 and 46.0 μM at pH 7.0, 6.5, 6.0, 5.5, 5.0 and 4.3, respectively. In the presence of 0.1, 0.2, 0.3 M KCl, at pH 6.0 and 25°C, the complex dissociation constants were 8.8, 20.0, 18.0 μM, respectively. The protein-dye complex dissociation constants show that Orange ZT could be used as an affinity ligand for protein purification. [...]
Publisher

Journal
Year
Volume
6
Issue
4
Pages
509-512
Physical description
Dates
published
1 - 12 - 2008
online
28 - 10 - 2008
Contributors
  • Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania, sjolanta@fm.vgtu.lt
  • Department of Chemistry and Bioengineering, Vilnius Gediminas Technical University, LT-10223, Vilnius-40, Lithuania
References
  • [1] M. Fountoulakis, J.-F. Juranville, L. Jiang, D. Avila, D. Roder, P. Jakob, P. Berndt, S. Evers, H. Langen, Amino Acids. 27, 249 (2004) http://dx.doi.org/10.1007/s00726-004-0141-1[Crossref]
  • [2] D.A. Colantonio, C. Dunkinson, D.E. Bovenkamp, J.E. Van Eyk, Proteomics 5, 3831 (2005) http://dx.doi.org/10.1002/pmic.200401235[Crossref]
  • [3] A. Tuncel, A. Denizli, D. Purvis, C.R. Lowe, J. Chromatogr. 634, 161 (1993) http://dx.doi.org/10.1016/0021-9673(93)83002-A[Crossref]
  • [4] E.B. Altintas, A. Denizli, J. Chromatogr. B Analyt. Technol. Biomed. Life Sci. 832, 216 (2006) http://dx.doi.org/10.1016/j.jchromb.2006.01.006[Crossref]
  • [5] S. Najarian, B.J. Bellhouse, Biomed. Sci. Instrum. 36, 153 (2000)
  • [6] A. Kassab, H. Yavuz, M. Odabasi, A. Denizli, J. Chromatogr. B Biomed. Sci. Appl. 746, 123 (2000) http://dx.doi.org/10.1016/S0378-4347(00)00311-X[Crossref]
  • [7] T. Zhang, H. Liu, J. Chen, Biotechnol. Prog. 15, 1078 (1999) http://dx.doi.org/10.1021/bp990113q[Crossref]
  • [8] A. Compagnini, S. Fisichella, S. Foti, G. Maccarrone, R. Saletti, J. Chromatogr. 736, 115 (1996) http://dx.doi.org/10.1016/0021-9673(95)01362-8[Crossref]
  • [9] M. Dockal, D.C. Carter, F. Ruker, J. Biol. Chem. 274, 29303 (1999) http://dx.doi.org/10.1074/jbc.274.41.29303[Crossref]
  • [10] J. Sereikaite, Z. Bumeliene, V.-A. Bumelis, Acta Chromatogr. 15, 298 (2005)
  • [11] G.B. Stopa, J. Rybarska, A. Drozd, L. Konieczny, M. Krol, M. Lisowski, B. Piekarska, I. Roterman, P. Spolnik, G. Zemanek, Int. J. Biol. Macromol. 40, 1 (2006) http://dx.doi.org/10.1016/j.ijbiomac.2006.05.002[Crossref]
  • [12] Zh. Bumeliene, J. Sereikaite, V.A. Bumelis, V. Smirnovas, G. Gedminiene, L. Braziunaite, E. Bajorunaite, J. Anal. Chem. 58, 1038 (2003) http://dx.doi.org/10.1023/A:1027325120597[Crossref]
  • [13] J. Sereikaite, V.-A. Bumelis, Biomed. Chromatogr. 20, 195 (2006) http://dx.doi.org/10.1002/bmc.552[Crossref]
  • [14] S.S. Flaksaite, O.F. Sudziuviene, J.H. Pesliakas, A.A. Glemza, Prikl. Biochim. Microb. 21, 25 (1985)
  • [15] M. Dockal, D.C. Carter, F. Ruker, J. Biol. Chem. 275, 3042 (2000) http://dx.doi.org/10.1074/jbc.275.5.3042[Crossref]
  • [16] J.F. Foster In: V.M. Rosenoer, M. Oratz, and M.A. Rothschild (Eds), Albumin structure, function and uses (Pergamon Press, Oxford, 1977) 53
  • [17] V. Vetri, F. Librizzi, M. Leone, V. Militello, Eur. Biophys. J. 36, 717 (2007) http://dx.doi.org/10.1007/s00249-007-0196-5[Crossref]
  • [18] I. Sutkeviciute, J. Sereikaite, V.-A. Bumelis, Biomed. Chromatogr. 22, 1001 (2008) http://dx.doi.org/10.1002/bmc.1019[Crossref]
  • [19] S. Angal, P.D.G. Dean, In: S. Roe (Ed), Protein purification techniques (Oxford University Press Inc., New York, 2001) 213
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-008-0066-8
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