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Journal
2008 | 6 | 3 | 410-418
Article title

Determination of phenolic acids by capillary zone electrophoresis and HPLC

Content
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Languages of publication
EN
Abstracts
EN
Selected phenolic acids are determined by capillary zone electrophoresis and HPLC, each using UV detection. The optimised CZE background electrolyte contained 50 mM acetic acid, 95 mM 6-aminocaproic acid, 0.1% polyacrylamide, 1% polyvinylpyrrolidone, and 10% methanol. Twelve phenolic acids (gallic, p-hydroxybenzoic, 3,4-dihydroxybenzoic, vanillic, syringic, o-coumaric, p-coumaric, caffeic, sinapic, ferulic, salicylic and chlorogenic) were separated within 10 minutes. Chromatographic separation of these phenolic acids was carried out on an Eclipse XBD C8 column using a mobile phase gradient (acetonitrile / methanol / water / 0.1% phosphoric acid); all were separated within 25 minutes. Electrophoretic and chromatographic determinations of ferulic and chlorogenic acids were compared on barley, malt, and potato samples. The methods’ characteristics were: linearity (1–20 mg ml and 0.2–4 mg ml−1), accuracy (recovery 94 ± 5% and 96 ± 4%), intra-assay repeatability (4.1% and 3.5%), and detection limit (0.2 and 0.02 mg ml−1). [...]
Publisher

Journal
Year
Volume
6
Issue
3
Pages
410-418
Physical description
Dates
published
1 - 9 - 2008
online
11 - 8 - 2008
Contributors
  • Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic, kvasnicf@vscht.cz
  • Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic
author
  • Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technická 3, Prague 6, Czech Republic
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-008-0032-5
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