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2008 | 6 | 2 | 222-228
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Spectrofluorometric determination of nicardipine, nifedipine and isradipine in pharmaceutical preparations and biological fluids

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A simple and highly sensitive spectrofluorometric method was developed for the determination of some 1,4-dihydropyridine compounds namely, nicardipine, nifedipine and isradipine in pharmaceutical preparations and biological fluids. The method is based on the reduction of nicardipine, nifedipine and isradipine with Zn/HCl and measuring the fluorescence intensity obtained (λem/λex) at 460/364, 450/393 and 446/360 nm, respectively. The factors affecting the development of the fluorophore and its stability were studied and optimized. The effect of some surfactants such as β-cyclodextrin (βCD), carboxymethylcelullose (CMC), sodium dodecyl sulphate (SDS) and triton X-100, on the fluorescence intensity was studied. The fluorescence intensity-concentration plots of nicardipine, nifedipine and isradipine were rectilinear over the ranges 0.4–6.0, 0.2–4.0 and 0.1–9.0 μg ml−1 with detection limits of 0.0028, 0.017 and 0.016 μg ml−1, respectively. The proposed method was successfully applied to commercial tablets containing the compounds; the percentage recovery agreed well with those obtained using the official methods. The method was further extended to the in vitro determination of the compounds in spiked human plasma and urine samples. A proposal of the reduction reaction pathway was postulated. [...]
Physical description
1 - 6 - 2008
17 - 4 - 2008
  • Girls College of Science, Department of Chemistry, P.O.Box 838, Dammam-31113, Saudi Arabia
  • Girls College of Science, Department of Chemistry, P.O.Box 838, Dammam-31113, Saudi Arabia
  • [1] F.E. Silvestry, M.G.St.J. Sutton, Eur. Heart J. 19 (Suppl. I), 8(1998)
  • [2] A. Goodman Gilman, T.W. Rall, A.S. Nies, P. Taylor, The Pharmacological Basis of Therapeutics, 8th edition (McGraw-Hill, New York, 1992)
  • [3] T. Godfraind, S. Salomone, J. Cardiovasc. Pharmacol, 30 (Suppl. 2), S1 (1997)
  • [4] F. Belal, A.A. Al-Majed, S. Julkhuf, N.Y. Khalil, Pharmazie 58, 874 (2003)
  • [5] C. Yánez, R. Salazar, L.J. Núñez-Vergara, J.A. Squella, J. Pharm. Biomed Anal. 35, 51 (2004)[Crossref]
  • [6] N. Rahman, M.N. Hoda, IL Farmaco 57, 435 (2002)[Crossref]
  • [7] M.M. Ghoneim, A. Tawfik, P.Y. Khashaba, Anal. Bioanal. Chem. 375, 369 (2006)
  • [8] F. Belal, A. Al-Majed, S. Julkhuf:, J. Pharm. Biomed. Anal. 31, 989 (2003)[Crossref]
  • [9] S.A. Ozkan, Pharmazie 57, 503 (2002)
  • [10] G. Altiokka, D. Dougrukol-Ak, M. Tuncel, H.Y. Aboul-Enein, Arch. Pharm. (Weinheim) 335, 104 (2002)<104::AID-ARDP104>3.0.CO;2-Q[Crossref]
  • [11] A. Alvarez-Lueje, S. Pujol, L.J. Naranjo, L.J. Núñez-Vergara, J. AOAC Int. 85, 1247 (2002)
  • [12] A.B. Baranda, R.M. Jiménez, R.M. Alonso, J. Chromatogr. A 1031, 275 (2004)[Crossref]
  • [13] V.B. Patravale, V.B. Nair, S.P. Gore, J. Pharm. Biomed. Anal. 23, 623 (2000)[Crossref]
  • [14] A.F. El Walily, J. pharm. Biomed. Anal. 16, 21 (1997)[Crossref]
  • [15] D. Zendelovska, et al., J. Chromatgr. B 839, 85 (2006)[Crossref]
  • [16] W. Kang, H. Yun, K. Liu, K. Kwon, J. Shin, J. Chromatgr. B 805, 311 (2004)[Crossref]
  • [17] K. Javidnia, R. Miri, A. Jamalian, J. Pharm. Biomed. Anal. 40, 438 (2006)[Crossref]
  • [18] J. Bases, J. Bartos, Colorimetric and Fluorometric Determination of Organic Compounds and Drugs, (Marcel Dekker, New York, 1974)
  • [19] T.W.G. Solomons, Organic chemistry, 6th edition (John Wiley & Sons, New York, 1996)
  • [20] H.H. Willard, L.L. Merritt, J.A.Jr. Dean, F.A.Jr. Settle, Instrumental Methods of Analysis, 7th Edition (CBS, New Delhi, 1986)
  • [21] J.C. Miller, J.N. Miller, Statistics for Analytical Chemistry, 4th edition (John Wiley & Sons, New York, 2000)
  • [22] The British Pharmacopoeia, HMSO, (The Pharmaceutical Press, London, 2004)
  • [23] A.A. Mustafa, K.A. Al-Rashood, M.E.M. Hagga, E.A. Gad-Kariem, M.E. Al-Awady, Pak. J. Sci. Ind. Res. 37,1 (1994)
  • [24] Y. Tokuma, H. Fujiwara, H. Noguchi, Biomed. Chromartogr. 14, 453 (2000)<453::AID-BMC5>3.0.CO;2-7[Crossref]
  • [25] A. Albini, E. Fasami, Drug Photochemistry and Photostability, (Royal Society of Chemistry, UK, 1998)
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