Mechanistic studies on the effect of veratryl alcohol on the lignin peroxidase catalyzed oxidation of pyrogallol red in reversed micelles

• Authors: Jing Lan , Xirong Huang , Ming Hu , Weifeng Liu , Yuezhong Li , Yinbo Qu , Peiji Gao
• Languages of publication: EN

Abstracts

EN

The lignin peroxidase (LiP) catalyzed oxidation of pyrogallol red (PR) in the absence and presence of veratryl alcohol (3,4-dimethoxybenzyl alcohol, VA) was carried out in bis (2-ethylhexyl) sulfosuccinate sodium (AOT)/ polyoxyethylene lauryl ether (Brij30) reversed micelles to elucidate the role of VA. Results indicated that VA could accelerate the LiP catalyzed oxidation of PR, especially at low H2O2 concentrations. Unlike in bulk aqueous medium, the protection of LiP by VA in the present medium was relatively unsubstantial, even at high H2O2 concentrations. Analysis of data from a series of experiments showed that the enhancement of the PR oxidation caused by VA was mainly due to the indirect oxidation of PR by VA+∙ from the LiP catalyzed oxidation of VA. It was also found that at the same protector concentration (40 μM), VA (the physiological substrate of LiP) was less effective than PR (a phenolic compound) in protecting LiP from the H2O2 derived inactivation. This novel phenomenon deserves further study. [...]

Keywords

EN

Lignin peroxidase; reversed micelle; veratryl alcohol; pyrogallol red; mediated oxidation

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2007
• Volume: 5
• Issue: 3
• Pages: 672-687

Dates

published

1 - 9 - 2007

online

9 - 6 - 2007

Contributors

author

Jing Lan

• Key Laboratory of Colloid Interface Chemistry of the Education Ministry of China, Shandong University, Jinan, 250100, P.R. China

author

Xirong Huang

author

Ming Hu

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

author

Weifeng Liu

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

author

Yuezhong Li

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

author

Yinbo Qu

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

author

Peiji Gao

• State Key Laboratory of Microbial Technology of China, Shandong University, Jinan, 250100, P.R. China

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Identifiers

DOI

10.2478/s11532-007-0032-x