PL EN


Preferences help
enabled [disable] Abstract
Number of results
Journal
2007 | 5 | 1 | 191-200
Article title

Oligonucleotides bearing pentaerythritol-derived mass tags

Content
Title variants
Languages of publication
EN
Abstracts
EN
Five different di-O-alkylated pentaerythritol phosphoramidite building blocks (2a–e) were synthesized and introduced into oligonucleotides to obtain mass-tagged probes (6a–f) useful in RNA transcription profiling. These non-nucleosidic mass tags allow categorization of oligonucleotide probes having identical nucleoside content and, hence, identification of the probe hybridized to RNA by mass spectrometry analysis. Hybridization properties of the oligonucleotide conjugates were elucidated by melting temperature measurements.
Publisher

Journal
Year
Volume
5
Issue
1
Pages
191-200
Physical description
Dates
published
1 - 3 - 2007
online
1 - 3 - 2007
Contributors
author
  • Department of Chemistry, University of Turku, FIN-20014, Turku, Finland
  • Department of Chemistry, University of Turku, FIN-20014, Turku, Finland
  • Department of Chemistry, University of Turku, FIN-20014, Turku, Finland, harlon@utu.fi
References
  • [1] J.D. Hoheisel: “Oligomer-chip technology”, Trends Biotechnol., Vol. 15, (1997), pp. 465–469. http://dx.doi.org/10.1016/S0167-7799(97)01123-2[Crossref]
  • [2] D.J. Duggan, M. Bittner, Y. Chen, P. Meltzer and J.M. Trent: “Expression profiling using cDNA microarrays”, Nature Gen., Vol. 21(Suppl.), (1999), pp. 10–14. http://dx.doi.org/10.1038/4434[Crossref]
  • [3] D.D.L. Bowtell: “Options available-from start to finish-for obtaining expression data by microarray”, Nature Gen., Vol. 21(Suppl.), (1999), pp. 25–32. http://dx.doi.org/10.1038/4455[Crossref]
  • [4] C.B. Epstein and R.A. Butow: “Microarray technology-enhanced versatility, persistent challenge”, Curr. Opin. Biotechnol., Vol. 11, (2000), pp. 36–41. http://dx.doi.org/10.1016/S0958-1669(99)00065-8[Crossref]
  • [5] A. Jung: “DNA chip technology”, Anal. Bioanal. Chem., Vol. 372, (2002), pp. 41–42. http://dx.doi.org/10.1007/s00216-001-1161-2[Crossref]
  • [6] A.-C. Syvänen, M. Laaksonen and H. Söderlund: “Fast quantification of nucleic acid hybrids by affinity-based hybrid collection”, Nucleic Acids Res., Vol. 14, (1986), pp. 5037–5048. http://dx.doi.org/10.1093/nar/14.12.5037[Crossref]
  • [7] A.-C. Syvänen, M. Bengtström, J. Tenhunen and H. Söderlund: “Quantification of polymerase chain reaction products by affinity-based hybrid collection”, Nucleic Acids Res., Vol. 16, (1988), pp. 11327–11338. http://dx.doi.org/10.1093/nar/16.23.11327[Crossref]
  • [8] For the utilization of mass-tagged oligonucleotides in genetic analysis, see a) T.J. Griffin, W. Tang and L.M. Smith: “Genetic analysis by peptide nucleic acid affinity MALDI-TOF mass spectrometry”, Nat. Biotechnol., Vol. 15, (1997), pp. 1368–1372 [Crossref]
  • [9] M. Kokoris, K. Dix, K. Moynihan, J. Mathis, B. Erwin, P. Grass, B. Hinesand and A. Duesterhoeft: “High-throughput SNP genotyping with the masscode system”, Molecular Dïagnosis, Vol. 5, (2000), pp. 329–340 http://dx.doi.org/10.1054/modi.2000.19218[Crossref]
  • [10] F.A. Abdi, M. Mundt, N. Doggett, E.M. Bradbury and X. Chen: “Validation of DNA sequences using mass spectrometry coupled with nucleoside mass tagging”, Genome Res., Vol. 12, (2002), pp. 1135–1141 http://dx.doi.org/10.1101/gr.221402[Crossref]
  • [11] W.T. Berggren, T. Takova, M.C. Olson, P.S. Eis, R.W. Kwiatkowski and L.M. Smith: “Multiplexed gene expression analysis using the invader RNA assay with MALDI-TOF mass spectrometry detection”, Anal. Chem., Vol. 74, (2002), pp. 1745–1750. http://dx.doi.org/10.1021/ac011167t[Crossref]
  • [12] C.H. Issidorides and R. Gulen: “Monobenzalpentaerythritol”, Org. Synth. Coll., Vol. IV, (1963), pp 679–681.
  • [13] P. Heinonen, P. Virta and H. Lönnberg: “Synthesis of Achiral α,α-Disubstituted β-Alanines, and Their Use in Construction of Libraries of β-Peptide Conjugates of N2-Alkyl-1,2,3,4-tetrahydroisoquinolines on Solid Support”, Tetrahedron. Vol. 55, (1999), pp. 7613–7624. http://dx.doi.org/10.1016/S0040-4020(99)00378-6[Crossref]
Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_s11532-006-0046-9
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.