Sequencing peptides by electrospray ion-trap mass spectrometry: A useful tool in synthesis of Axinastatin 3

• Authors: Chenxi Jia , Wei Qi , Zhimin He , Bin Qiao
• Languages of publication: EN

Abstracts

EN

Axinastatin 3 as a potential anticancer agent was synthesized by chemical methods. In an electrospray ion-trap mass spectrometer, using one stage of tandem mass spectrometry (MS/MS), the linear peptide intermediate was sequenced via the complementarities of y and b ions. Then, using multistep MS/MS (to MS6), the cyclic peptide was sequenced through sequentially removing one amino acid residue in each stage of MS/MS. The difference of the fragmentation mechanisms and the sequencing approaches between them is discussed.

Keywords

EN

Tandem mass spectrometry; cyclic peptides; fragmentation pathways; collisionally activated decomposition

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2006
• Volume: 4
• Issue: 4
• Pages: 620-631

Dates

published

1 - 12 - 2006

online

1 - 12 - 2006

Contributors

author

Chenxi Jia

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

author

Wei Qi

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

author

Zhimin He

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

author

Bin Qiao

• Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

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Identifiers

DOI

10.2478/s11532-006-0028-y