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Number of results

Journal

2006 | 4 | 2 | 285-298

Article title

Synthesis of heptapeptides and analysis of sequence by tandem ion trap mass spectrometry

Content

Title variants

Languages of publication

EN

Abstracts

EN
Two heptapeptides have been prepared by Fmoc methodology using Wang resin as solid support. For attachment of the first amino acid, several coupling systems were evaluated, and DIC/DMAP system could give yields of >99% and low levels of racemization. The selection of scavenger combination to deprotect side chains revealed that H2O/p-cresol was good at scavenging trityl and 1,2-ethanedithiol was highly efficient for scavenging t-butyl. Through shortening the preactivation time to 5 min, the racemization which occurred during formation of amide bonds coupled by HBTU was minimized. The crude peptides were characterized by RP-HPLC and MS, and sequenced by MS/MS to acquire reliable amino acid sequence information.

Publisher

Journal

Year

Volume

4

Issue

2

Pages

285-298

Physical description

Dates

published
1 - 6 - 2006
online
1 - 6 - 2006

Contributors

author
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
author
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
author
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
author
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China
author
  • Chemical Engineering Research Center, School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, People’s Republic of China

References

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  • [21] Y.S. Zou and X.Z. Qian: “Test of the amino group of amino acid and polypeptides on solid phase”, Prog. Biochem. Biophys., Vol. 14, (1978), pp. 12–14.
  • [22] A. Grandas, X. Jorba, E. Giralt and E. Pedroso: “Anchoring of Fmoc-amino acids to hydroxylmethyl resins”, Int. J. Peptide Protein Res., Vol. 33, (1989), pp. 386–390.

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_2478_s11532-006-0004-6
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