Analysis of Genetic Similarities Between Clostridium Perfringens Isolates Isolated from Patients with Gas Gangrene and from Hospital Environment Conducted with the use of the PFGE Method

• Authors: Monika Brzychczy-Włoch , Małgorzata Bulanda
• Languages of publication: EN

Abstracts

EN

The objective of the study was to perform a comparative analysis of genetic similarity, with the use of pulsed field gel electrophoresis (PFGE), of Clostridium perfringens isolates originating from patients with gas gangrene and from the hospital environment. The study encompassed two patients with a clinical and microbiological diagnosis of gas gangrene, who were hospitalized in one of the hospitals of the Małopolska province in the time period between 31st March 2012 and 18th May 2012. Clostridium perfringens isolates genotyping indicated that the isolates originating from the two studied patients did not display genetic similarity and represented two different PFGE types, which corresponded to two different clones (clone A and B). Whereas the strains isolated from the hospital environment were genetically identical with the strain coming from the second patient and represented one PFGE type, which corresponded to one clone (clone A). As a result of the study, it is possible to conclude that both patients developed endogenous infection. Even so, the examination of the hospital environment indicates the possibility of the appearance of exogenous infections. It prompts recommending and following the exact regulations of sanitary regime in the ward and the operating theater if a patient is diagnosed with gas gangrene.

Keywords

EN

Clostridium perfringens; gas gangrene; pulsed field gel electrophoresis - PFGE; epidemiological investigation

• Publisher: De Gruyter Open
• Journal: Polish Journal of Surgery
• Year: 2015
• Volume: 86
• Issue: 3
• Pages: 141-146

Dates

published

1 - 3 - 2014

online

25 - 4 - 2014

received

4 - 10 - 2013

Contributors

author

Monika Brzychczy-Włoch

• Department of Bacteriology, Microbial Ecology and Parasitology, Chair of Microbiology, Jagiellonian University Medical College in Cracow

author

Małgorzata Bulanda

• Department of Infection Epidemiology, Chair of Microbiology, Jagiellonian University Medical College in Cracow

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Identifiers

DOI

10.2478/pjs-2014-0026