CIDE-A gene expression in patients with abdominal obesity and LDL hyperlipoproteinemia qualified for surgical revascularization in chronic limb ischemia

• Authors: Marcin Feldo , Janusz Kocki , Sylwia Łukasik , Jacek Bogucki , Jan Feldo , Piotr Terlecki , Jan Kęsik , Jacek Wroński , Tomasz Zubilewicz
• Languages of publication: EN

Abstracts

EN

According to the latest data, CIDE -A gene plays a key role in the regulation of body weight in both humans and mice, and therefore it is regarded a potential candidate gene for human obesity. The aim of the study was to define the role of CIDEA gene in patients with dyslipidemia and symptomatic limb ischemia. Material and methods. The study group contained 28 patients, including 17 men and 11 women. Patients were enrolled in the study group, depending on the value of body mass index (BMI); there was BMI>30 for obese patients. The group included untreated patients (n=14) and patients (n=14) receiving atorvastatin 20 mg/day for at least three months prior to the initiation of the study. The control group (n=16) contained patients with no lipid disorders. A one-step isolation of RNA from lymphocytes and adipose tissue cells was carried out using the TRI method modified by Chomczyński and Sacchi. Next, gene expression was tested using real-time PCR. Results. The highest mean relative expression of CIDE -A gene occurred in patients with normal body weight. The lowest mean relative expression of CIDE-A gene was observed in obese patients with lipid disorders. A high negative correlation (r=-0.7919) of CIDE -A gene expression, depending on BMI, was reported in the group of obese patients with lipid disorders. Conclusions. Due to an important role of Cide-A protein demonstrated in the development of metabolic diseases such as obesity, metabolic syndrome, type 2 diabetes and their vascular complications, CIDE -A gene and protein are potential therapeutic targets in the case of these diseases.

Keywords

EN

CIDE-A gene; obesity; femoro-popliteal revascularisation; dyslipidemia

• Journal: Polish Journal of Surgery
• Year: 2013
• Volume: 85
• Issue: 11
• Pages: 644-648

Dates

published

1 - 11 - 2013

online

31 - 12 - 2013

Contributors

author

Marcin Feldo

• Department of Vascular Surgery and Angiology, Medical University in Lublin

author

Janusz Kocki

• Department of Clinical Genetics, Medical University in Lublin

author

Sylwia Łukasik

• Department of Cardiology, Medical University in Lublin

author

Jacek Bogucki

• Department of Pedeutology and Health Education, Maria Skłodowska-Curie University in Lublin

author

Jan Feldo

• Department of Psychiatry, Neuropsychiatric District Hospital in Lublin

author

Piotr Terlecki

• Department of Vascular Surgery and Angiology, Medical University in Lublin

author

Jan Kęsik

• Department of Vascular Surgery and Angiology, Medical University in Lublin

author

Jacek Wroński

• Department of Vascular Surgery and Angiology, Medical University in Lublin

author

Tomasz Zubilewicz

• Department of Vascular Surgery and Angiology, Medical University in Lublin

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Identifiers

DOI

10.2478/pjs-2013-0097