Future Applications of Apricot (Prunus Armeniaca Kaisa) ß Galactosidase in Dairy Industry

• Authors: Shakeel Ahmed Ansari , Rukhsana Satar , Syed Kashif Zaidi , Mohd Jahir Khan , Muhammad Imran Naseer , Mohammed H. Al-Qahtani , Mohamad Yusof Maskat
• Languages of publication: EN

Abstracts

EN

The present study demonstrates the immobilization of β galactosidase from apricots (Prunus armeniaca kaisa) on an inexpensive concanavalin A layered cellulose-alginate hybrid gel. Immobilized β galactosidase retained 78% of the initial activity after crosslinking by glutaraldehyde. It exhibited greater fraction of activity at both acidic and basic pH, and showed broad spectrum temperature optimum as compared to free enzyme. Moreover, immobilized enzyme exhibited higher thermal stability at 60°C and retained 80% of the original enzyme activity in presence of 3% galactose. The crosslinked immobilized enzyme showed improved hydrolysis of lactose from milk and whey in batch processes at 50°C as well as in continuous reactors operated at fl ow rate of 20 mL/h and 30 mL/h even after one month. Moreover, crosslinked adsorbed β galactosidase retained 76% activity even after its sixth repeated use, thereby promoting its use for lactose hydrolysis in various dairy products even for longer durations.

Keywords

EN

Apricots; β galactosidase; lactose hydrolysis; dairy industries

• Publisher: De Gruyter Open
• Journal: Polish Journal of Chemical Technology
• Year: 2014
• Volume: 16
• Issue: 3
• Pages: 74-79

Dates

published

1 - 9 - 2014

online

3 - 10 - 2014

Contributors

author

Shakeel Ahmed Ansari

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

author

Rukhsana Satar

• Ibn Sina National College for Medical Studies, Department of Biochemistry, Jeddah-21418, Saudi Arabia

author

Syed Kashif Zaidi

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

author

Mohd Jahir Khan

• Universiti Kebangsaan Malaysia, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, 43600 Bangi, Selangor Darul Ehsan, Malaysia

author

Muhammad Imran Naseer

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

author

Mohammed H. Al-Qahtani

• King Abdulaziz University, Center of Excellence in Genomic and Medicine Research, Jeddah-21589, Saudi Arabia

author

Mohamad Yusof Maskat

• Universiti Kebangsaan Malaysia, School of Chemical Sciences and Food Technology, Faculty of Science and Technology, 43600 Bangi, Selangor Darul Ehsan, Malaysia

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Identifiers

DOI

10.2478/pjct-2014-0054