PL EN


Preferences help
enabled [disable] Abstract
Number of results
2014 | 1 | 1 |
Article title

Matrix-mediated regulation of Ca2+ oscillations in osteodifferentiation of human mesenchymal stem
cells

Content
Title variants
Languages of publication
EN
Abstracts
EN
Human mesenchymal stem cells (hMSCs) have
been demonstrated to exhibit characteristic cytosolic Ca2+ ([Ca2+]i) oscillations in response to physicochemical factors,
including soluble factors, matrix proteins and microenvironment.
It has been postulated that the calcium
oscillation at the early stages of hMSC differentiation may
serve as a biophysical predictor. The goal of the present
study is therefore aimed at elucidating the matrix proteinmediated
[Ca2+]i oscillation that occurs in the early stages
of hMSC osteogenic differentiation. On the collagen substrates
of different stiffness and concentrations, intracellular
[Ca2+]i oscillations of hMSCs exhibited rapid spikes
on the hard substrates and the opposite on the soft substrate.
Cells seeded on a hard substrate also expressed signi
ficantly high levels of osteogenic gene markers, suggesting
a key role of intracellular calcium in the transduction
of ECM signaling to the hMSC differentiation. Our findings
revealed that the characteristics of [Ca2+]i oscillation
are regulated by the biochemical and mechanical properties
of the collagen substrate. Furthermore, β1-integrin
and associated signaling proteins found in focal adhesions
(FAs) were involved in the signaling pathways. Because
the [Ca2+]i signaling and stemcell differentiation appear
closely correlated, the regulation of [Ca2+]i signaling
through the modification of engineeredECMmay provide a
controllable exogenous technique to direct the fate of hMSCs.
Publisher
Year
Volume
1
Issue
1
Physical description
Dates
received
16 - 1 - 2014
accepted
20 - 2 - 2014
online
30 - 6 - 2014
References
  • [1] Altieri D.C., Stamnes S.J. and Gahmberg C.G., Regulated Ca2+signaling through leukocyte CD11b/CD18 integrin. Biochem. J.1992; 288: 465-473
  • [2] Chang H.-H., Lin C.-H., Lo S.J., Recombinant rhodostomin substratesinduce transformation and active calcium oscillation inhuman plates, Experimental Cell Res. 1999; 250: 387-400
  • [3] Creecy C.M., O’Neill C.F., Arulanandam B.P., Sylvia V.L., NavaraC.S., Bizios R., Mesenchymal stem cell osteodifferentiationin response to alternating electric current. Tissue Eng. Part A.2013; 9:467-74.[WoS][Crossref]
  • [4] Den Dekker E., Gorter G., van der Vuurst H., Heemskerk J.W.,Akkerman J.W., Biogenesis of G-protein mediated calciumsignaling in human megakaryocytes. Thromb Haemost 2001;86: 1106-13
  • [5] D’Souza S.J., Pajak A., Balazsi K., Dagnino L., (2001) Ca2+ andBMP-6 signaling regulate E2F during epidermal keratinocytedifferentiation. J. Biol. Chem. 276, 23531-8
  • [6] Du M., Liang H., Mou C., Li X., Sun J., Zhuang Y., Xiao Z., ChenB., Dai J., Regulation of human mesenchymal stem cells differentiationinto chondrocytes in extracellular matrix-basedhydrogel scaffolds. Colloids Surf. B Biointerfaces. 2013;114C:316-323[WoS][PubMed]
  • [7] Ducibella T., Huneau D., Angelichio E., Xu Z., Schultz R.M.,Kopf G.S., Fissore R., Madoux S., Ozil J.P., Egg-to-embryo transitionis driven by differential responses to Ca2+ oscillationnumber. Dev. Biol. 2002; 250: 280-91
  • [8] Engler A.J., Sen S., Sweeney H.L. and Discher D.E., Matrixelasticity directs stem cell lineage specification. Cell2006;126,:677-689
  • [9] Francesconi M., Casonato A., Pontara E., Dalla V.L., GirolamiA., Deana R., Type IIB von Willebrand factor induces phospholipaseA2 activation and cytosolic Ca2+ increase in platelets.Biochem. Biophys. Res. Commun. 1995; 214: 102-109
  • [10] Giannone G., Rondé P., Gaire M., Beaudouin J., Haiech J., EllenbergJ., and Takeda K., Calcium rises locally trigger gocaladhesion disassembly and enhance residency of focal adhesionkinase at focal adhesions. The Journal of BiologicalChemistry 2004; 279: 28715-28723
  • [11] Giannone G., Ronde P., Gaire M., Haiech J., Takeda K., Calciumoscillations trigger focal adhesion disassembly in human U87astrocytoma cells. The Journal of Biological Chemistry 2002;277: 26364-26371
  • [12] Gkretsi V., Bowen W.C., Yang Y., Wu C., Michalopoulos G.K.,Integrin-linked kinase is involved in matrix-induced hepatocytedifferentiation. Biochemical and Biophysical ResearchCommunications 2007; 353: 638-643[WoS]
  • [13] Hellberg C., Eierman D., Sjolander A. and Andersson T., TheCa2+ signaling capacity of the beta 2-integrin on HL-60 granulocyticcells is abrogated following phosphorylation of its CD18-chain: relation to impaired protein tyrosine phosphoorylation.Exp. Cell Res. 1995; 217: 140-148
  • [14] Jones K.T., Ca2+ oscillations in the activation of the egg anddevelopment of the embryo in mammals. Int. J. Dev. Biol.1998; 42: 1-10
  • [15] Katz E., Streuli C.H., The extracellular matrix as an adhesioncheckpoint for mammary epithelia function. Int. J. Biochem.Cell Biol. 2007; 39: 715-726[Crossref][WoS]
  • [16] Kawano S., Shoji S., Ichinose S., Yamagata K., Tagami M.,Hiraoka M., Characterization of Ca2+ signaling pathways inhuman mesenchymal stem cells. Cell Calcium 2002; 32: 165-174[PubMed][Crossref]
  • [17] Kimura K., Yanagida Y., Haruyama T., Kobatake E., Aizawa M.,Gene expression in the electrically stimulated differentiationof PC12 cells. Journal Biotechnology 1998; 63: 55-65
  • [18] Klumpers D.D., Zhao X., Mooney D.J., Smit T.H., Cell mediatedcontraction in 3D cell-matrix constructs leads to spatially regulatedosteogenicdifferentiation. Integr. Biol. (Camb). 2013;5:1174-83[WoS]
  • [19] Lehmann U. and Kreipe H., Real-Time PCR Analysis of DNA andRNA Extracted from Formalin-Fixed and Paraffin-EmbeddedBiopsies. Methods 2001; 25: 409-418[Crossref][PubMed]
  • [20] Li Y.J., Batra N.N., You L., Meier S.C., Coe I.A., Yellowley C.E.,Jacobs C.R., Oscillatory fluid flow affects human marrow stromalcell proliferation and differentiation. J. Orthop. Res. 2004;22: 1283-1289[Crossref]
  • [21] Marhl M., Haberichter T., Brumen M., Heinrich R., (2000) Complexcalcium oscillations and the role of mitochondria andcytosolic proteins. BioSystems 2000; 57: 75-86
  • [22] McAinsh M.R., Hetherington A.M., Encoding specificity in Ca2+signaling systems. Trends Plant Sci. 1998; 3: 32-36[Crossref]
  • [23] McNamee H.P., Ingber D.E. and Schwartz M.A., Adhesion tofibronectin stimulates inositol lipid synthesis and enhancesPDGF-induced inositol lipid breakdown. J. Cell Biol. 1993: 121:673-678
  • [24] Poole A.W., Watson S.P., Regulation of cytosolic calcium bycollagen in single human platelets. Br. J. Pharmacol. 1995; 115:101-106
  • [25] Radmacher M., Measuring the elastic properties of living cellsby the atomic force microscope. Methods Cell Biol. 2002;68:67-90[PubMed][Crossref]
  • [26] Ronde P., Giannone G., Gerasymova I., Stoeckel H., TakedaK., Haiech J., Mechanism of calcium oscillations in migratinghuman astrocytoma cells. Biochimica et Biophysica Acta 2000;1498: 272-280
  • [27] Salaxznyk R.M., Williams W.A., Boskey A., Batorsky A., PlopperG.E., Adhesion to vitronectin and collagen I promotesosteogenic differentiation of human mesenchymal stem cells.Journal of Biomedicine and Biotechnology 2004; 2004, 23-34
  • [28] Sauer H., Rahimi G., Hescheler J., Wartenberg M., Effects ofElectrical Fields on Cardiomyocyte Differentiation of EmbryonicStem Cells, Journal of Cellular Biochemistry 1999; 75: 710–723[Crossref][WoS]
  • [29] Sjaastad M.D. and Nelson W.J., Integrin-mediated calcium signalingand regulation of cell adhesion by intracellular calcium.BioEssays 1997; 19: 47-55[Crossref][PubMed]
  • [30] Sjaastad M.D., Angres B., Lewis R.S., Nelson W.J., Feedbackregulation of cell-substratum adhesion by integrin-mediatedintracellular Ca2+ signaling. Proc. Natl. Acad. Sci. U S A. 1994;91:8214-8.[Crossref]
  • [31] Skardal A., Mack D., Atala A., Soker S., Substrate elasticitycontrols cell proliferation, surface marker expression andmotile phenotype in amniotic fluid-derived stem cells. J. Mech.Behav. Biomed. Mater. 2013;17: 307-16.[Crossref][WoS]
  • [32] Spitzer N., Activity-dependent neuronal differentiation priorto synapse formation: the functions of calcium transients.Journal of Physiology-Paris 2002; 96: 73-80[Crossref]
  • [33] Sun S., Liu Y.M., Lipsky S. and Cho M., Physical manipulationof calcium oscillations facilitates osteodifferentiation of human mesenchymal stem cells. FESAB J. 2007; 21: 1472-80[WoS][PubMed]
  • [34] Sun S., Titushkin I., Cho M., Regulation of mesenchymal stemcell adhesion and orientation in 3D collagen scaffold by electricalstimulus. Bioelectrochemistry 2006; 69: 133–141[Crossref][PubMed]
  • [35] Tang C., Xu Y., Jin C., Min B.H., Li Z., Pei X., Wang L., Feasibilityof Autologous Bone Marrow Mesenchymal Stem Cell-DerivedExtracellular MatrixScaffold for Cartilage Tissue Engineering.Artif Organs. 2013 Nov 20. doi: 10.1111/aor.12130[Crossref][WoS]
  • [36] Titushkin I., Cho M., Modulation of cellular mechanics duringosteogenic differentiation of human mesenchymal stem cells.Biophys. J. 2007; 93:3693–3702.[Crossref][PubMed]
  • [37] Torihashi S., Fujimoto T., Trost C. and Nakayama S., Calciumoscillation linked to pacemaking of interstitial cells of cajal.Journal of Biological Chemistry 2002; 277, 19191-19197
  • [38] Trewavas A.J., Malho R., Ca2+ signaling in plant cells: The bignetwork. Curr. Opin. Plant Biol. 1998; 1: 428-433[Crossref]
  • [39] Trinkaus-Randall V., Kewalramani R., Payne J. and Cornell-BellA., (2000) Calcium signaling induced by adhesion mediatedprotein tyrosine phosphorylation and is independent of pHi.Journal of Cellular Physiology 2000; 184: 385-399
  • [40] Yanagida E., Shoji S., Hirayama Y., Yoshikawa F., Otsu K., UematsuH., Hiraoka M., Furuichi T., Kawano S., Functional expressionof Ca2+ signaling pathways in mouse embryonic stemcells. Cell Calcium 2004; 36: 135-46[Crossref]
  • [41] Zhao M., Bai H., Wang E., Forrester J.V., McCaig C.D., Electricalstimulation directly induces pre-angiogenic responses in vascularendothelial cells by signaling through VEGF receptors. J.Cell Sci. 2004; 117: 397-405
Document Type
Publication order reference
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_pcsc-2014-0001
Identifiers
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.