Contrast-phase Imaging of Fixed-Cells through Micro-Cavity Scanning Microscopy

• Authors: Andrea Di Donato , T. Pietrangelo , T. Da Ros , Tamara Monti , Marco Farina
• Languages of publication: EN

Abstracts

EN

Contrast phase imaging at infrared wavelengths
is achieved through an extrinsic Fabry-Perot cavity in optical
fiber. The micro-cavity is realized by approaching a
cleaved fiber to a distance of about few tens of microns
from the surface under test. The probe is a single mode
fiber and is fed by a low-coherence source. The information
is extracted from the reflected spectrum, that starts
to be modulated by the interference when the fiber begins
to interact with the sample. The measurement of the
reflected optical intensity provides a map of the sample
reflectivity, whereas from the analysis of the spectrum in
the time/spatial domain, it is possible to extract topography
and refractive index variations. This information is
entangled in the contrast phase image obtained. In this
work we review the system proposed in [19] in order to
extract topography and local surface permittivity of biological
samples. The system displays tridimensional images
with a transverse resolution that is not limited by
the numerical aperture NA of the scanning probe (as suggested
by the Rayleigh limit), but it is related to the transverse
field behavior of the electromagnetic field inside the
micro-cavity. Differently, the source bandwidth, demodulation
algorithm and optical spectrum analyzer resolution
affect the resolution in the normal direction.

Keywords

EN

scanning microscopy; micro-cavity; lowcoherence interferometry

• Publisher: De Gruyter Open
• Journal: Optical Data Processing and Storage
• Year: 2014
• Volume: 1
• Issue: 1

Dates

accepted

11 - 9 - 2013

online

27 - 2 - 2014

received

9 - 5 - 2013

Contributors

author

Andrea Di Donato

• Dipartimento di Ingegneria
  dell’Informazione, Università Politecnica delle Marche,
  Ancona, 60131, Italy

author

T. Pietrangelo

• Dept. of Neuroscience and Imaging, Università “G.
  d’Annunzio’, Via dei Vestini, 66013, Chieti Italy

author

T. Da Ros

• Dip. di Scienze Chimiche e Farmaceutiche, Università di
  Trieste,Via Valerio, 34127, Trieste

author

Tamara Monti

• Dipartimento di Ingegneria
  dell’Informazione, Università Politecnica delle Marche,
  Ancona, 60131, Italy

author

Marco Farina

• Dipartimento di Ingegneria
  dell’Informazione, Università Politecnica delle Marche,
  Ancona, 60131, Italy

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Identifiers

DOI

10.2478/odps-2014-0003