The relationship between the alkaline phosphatase network and the haematopoiesis in mice subjected to whole-body irradiation
Languages of publication
Purpose: To investigate the relationship between the alkaline phosphatase (ALP) network of the marrow stroma and the haematopoietic regeneration after mice whole-body irradiation. Materials and methods: Three groups of mice were irradiated with a non-lethal ionising radiation dose: the fi rst one received an intraperitoneal injection of Levamisole, ALP inhibitor, 24 h before irradiation; the second one received an intraperitoneal injection of Lisinopril, haematopoiesis inhibitor, 24 h before irradiation; the third was left untreated, but irradiated. The fourth group, untreated and not irradiated, was the control. The total surface occupied by ALP positive processes, revealed by means of ALP cytochemistry in the marrow area, was evaluated semi-quantitively. Nucleated bone marrow cells were also counted. Results: ALP network began to increase 24 h after irradiation to reach a maximum after 72 h, when the bone marrow was almost become completely empty of the haematopoietic cells. This increase advances the haematopoietic recovery. This process was substantially delayed when the mice were injected with Levamisole 24 h before irradiation. On the contrary, ALP network increased strongly since the fi rst day after irradiation when the mice were injected with Lisinopril 24 h before irradiation. Conclusions: These data have indicated that the haematopoietic recovery and repopulation of the bone marrow were advanced by the ALP network recovery.
1 - 8 - 2014
11 - 6 - 2014
12 - 9 - 2014
20 - 2 - 2014
- Hematological Diseases Laboratory, Department of Radiation Medicine, Atomic Energy Commission of Syria (AECS), P. O. Box 6091 Damascus, Syria, Tel.: +963 11 213 2580, Fax: +963 11 611 2289, firstname.lastname@example.org
- Hematological Diseases Laboratory, Department of Radiation Medicine, Atomic Energy Commission of Syria (AECS), P. O. Box 6091 Damascus, Syria, Tel.: +963 11 213 2580, Fax: +963 11 611 2289
- 1. Mayani, H., Guilbert, L., & Janowska-Wieczorek, A. (1992). Biology of the hemopoietic microenvironment. Eur. J. Haematol., 49, 225-233.
- 2. Bianco, P., & Boyde, A. (1993). Confocal images of marrow stromal (Westen-Bainton) cells. Histochemistry, 100, 93-99.[Crossref][PubMed]
- 3. Kittler, E. L., McGrath, H., Temeles, D., Crittenden, R. B., Kister, V. K., & Quesenberry, P. J. (1992). Biologic signifi cance of constitutive and subliminal growth factor production by bone marrow stroma. Blood, 79, 3168-3178.
- 4. Long, H. W. (1992). Blood cell cytoadhesion molecules. Exp. Hematol., 20, 288-301.
- 5. Krenacs, T., & Rosendaal, M. (1998). Connexin 43 gap junctions in normal, regenerating and cultured mouse bone marrow and in human leukemia: their possible involvement in blood formation. Am. J. Pathol., 152, 993-1004.
- 6. Westen, H., & Baiton, D. F. (1979). Association of alkaline-phosphatase-reticulum cells in bone marrow with granulocytic precursors. J. Exp. Med., 150, 919-937.
- 7. McKenna, R. W., & Allison, P. (1990). Diagnosis, classifi cation, and course of myelodysplasic syndromes. Clin. Lab. Med., 10, 683-706.
- 8. Bianco, P., Bradbeer, J. N., Riminucci, M., & Boyde, A. (1993). Marrow stromal (Western-Bainton) cells: identifi cation, morphometry, confocal imaging and changes in disease. Bone, 14, 315-320.[Crossref]
- 9. Dilly, S. A., & Jagger, C. J. (1990). Bone marrow stromal cell changes in haematological malignancies. J. Clin. Pathol., 43, 942-947.
- 10. Almohamad, K., Thiry, A., Hubin, F., Belaid, Z., Humblet, C., Boniver, J., & Defresne, M. -P. (2003). Marrow stromal cell recovery after radiation-induced aplasia in mice. Int. J. Radiat. Biol., 79(4), 259-267.
- 11. Rousseau-Plasse, A., Wdzieczak-Bakala, J., Lenfant, M., Ezan, E., Robinson, S., Briscoe, C. V., Melville, J., & Riches, A. (1998). Lisinopril, an angiotensin I-converting enzyme inhibitor, prevents entry of murine hematopoietic stem cells into the cell cycle after irradiation in vivo. Exp. Hematol., 26, 1074-1079.
- 12. Fallon, M. D., Whyte, M. P., & Teitelbaum, S. L. (1980). Stereospecifi c inhibition of alkaline phosphatase by L-tetramisole prevents in vitro cartilage calcifi cation. Lab. Invest., 43, 489-494.
- 13. Scher, B. M., Fuksina, I., Hellinger, N., Waxman, S., & Scher, W. (1998). The phosphatase inhibitors, orthovanadate and levamisole, inhibit induction of erythroid differentiation and abrogate the associated inhibition of glycolysis. Int. J. Oncol., 12, 987-996.[PubMed]
- 14. Bianco, P., Costantini, M., Dearden, L. C., & Bonucci, E. (1988). Alkaline phosphatase positive precursors of adipocytes in the human bone marrow. Br. J. Haematol., 68, 401-403.
- 15. Prince, M. H., Simmons, P. J., Whitty, G., Wall, D. P., Barber, L., Toner, G. C., Seymoour, R. F., Richardson, G., Mrongovius, R., & Haylock, D. N. (2004). Improved hematopoietic recovery following transplantation with ex vivo-expanded mobilized blood cells. Br. J. Haematol., 126(4), 536-545.
- 16. Haot, J., & Barakina, N. F. (1969). Quantitative study of the hemopoietic recovery after a sublethal X-irradiation in the mouse. Acta Haematol., 42, 347-356.
- 17. Imai, Y., & Nakao, I. (1987). In vivo radiosensitivity and recovery pattern of the hematopoietic precursor cells and stem cells in mouse bone marrow. Exp. Hematol., 15, 890-895.[PubMed]
- 18. Brookoff, D., & Weiss, L. (1982). Adipocyte development and the loss of erythropoietic capacity in the bone marrow of mice after sustained hypertransfusion. Blood, 60, 1337-1344.
- 19. Dilly, S. A., Jagger, C. J., & Sloane, J. P. (1993). Stromal cell populations in necropsy bone marrow sections from allogeneic marrow recipients and non- -transplant patients. J. Clin. Pathol., 46, 611-616.
- 20. Poncin, G., Beaulieu, A., Humblet, C., Thiry, A., Oda, K., Boniver, J., & Defresne, M. -P. (2012). Characterization of spontaneous bone marrow recovery after sublethal total body irradiation: importance of the osteoblastic/adipocytic balance. PLoS One 7:e30818. [Crossref][WoS]
Publication order reference