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2014 | 1 | 1 |

Article title

Single-molecule FRET for Ultrasensitive Detection of Biomolecules

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Abstracts

EN
Single-molecule Förster resonance energy transfer (sm- FRET) has been widely employed to detect biomarkers and to probe the structure and dynamics of biomolecules. By monitoring the biological reaction in a spatio-temporal manner, smFRET can reveal the transient intermediates of biological processes that cannot be obtained by conventional ensemble measurements. This review provides an overview of singlemolecule FRET and its applications in ultrasensitive detection of biomolecules, including the major techniques and the molecular probes used for smFRET as well as the biomedical applications of smFRET. Especially, the combination of sm- FRET with new technologies might expand its applications in clinical diagnosis and biomedical research

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Journal

Year

Volume

1

Issue

1

Physical description

Dates

published
1 - 1 - 2014
accepted
10 - 10 - 2013
received
19 - 9 - 2013
online
29 - 11 - 2013

Contributors

author
  • Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China
author
  • Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China
  • Single-molecule Detection and Imaging Laboratory, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, 518055, China

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bwmeta1.element.-psjd-doi-10_2478_nbi-2013-0002
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