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2014 | 2 | 1 |
Article title

Fabrication and Characterization of Three
Dimensional Electrospun Cortical Bone Scaffolds

Content
Title variants
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EN
Abstracts
EN
Bone is a composite tissue composed of an
organic matrix, inorganic mineral matrix and water.
Structurally, bone is organized into two distinct types:
trabecular (or cancellous) and cortical (or compact) bone.
Cortical bone is highly organized, dense and composed of
tightly packed units or osteons whereas trabecular bone
is highly porous and usually found within the confines
of cortical bone. Osteons, the subunits of cortical bone,
consist of concentric layers of mineralized collagen
fibers. While many scaffold fabrication techniques have
sought to replicate the structure and organization of
trabecular bone, very little research focuses on mimicking
the organization of native cortical bone. In this study
we fabricated three-dimensional electrospun cortical
scaffolds by heat sintering individual osteon-like scaffolds.
The scaffolds contained a system of channels running
parallel to the length of the scaffolds, as found naturally
in the haversian systems of bone tissue. The purpose
of the studies discussed in this paper was to develop a
mechanically enhanced biomimetic electrospun cortical
scaffold. To that end we investigated the appropriate
mineralization and cross-linking methods for these
structures and to evaluate the mechanical properties
of scaffolds with varying fiber angles. Cross-linking the
gelatin in the scaffolds prior to the mineralization of the scaffolds proved to help prevent channels of the osteons
from collapsing during fabrication. Premineralization,
before larger scaffold formation and mineralization,
increased mineral deposition between the electrospun
layers of the scaffolds. A combination of cross-linking and
premineralization significantly increased the compressive
moduli of the individual scaffolds. Furthermore, scaffolds
with fibers orientation ranging between 15° and 45° yielded
the highest compressive moduli and yield strength.
Publisher

Year
Volume
2
Issue
1
Physical description
Dates
online
12 - 11 - 2014
accepted
15 - 4 - 2014
received
17 - 2 - 2014
Contributors
author
  • Virginia Tech-Wake Forest University,
    School of Biomedical Engineering and Sciences, Blacksburg, VA,
    USA 24061
  • Virginia Tech-Wake Forest University,
    School of Biomedical Engineering and Sciences, Blacksburg, VA,
    USA 24061
  • University of Virginia, Department of Biomedical
    Engineering, Charlottesville, VA
  • Virginia Tech-Wake Forest University,
    School of Biomedical Engineering and Sciences, Blacksburg, VA,
    USA 24061
  • Virginia Tech, Material Science and Engineering,
    Blacksburg, VA
  • Virginia Tech, Department of Chemical Engineering,
    Blacksburg, VA
  • Rutgers University,
    Department of Biomedical Engineering, Piscataway, NJ
  • Dominion University School of Medical Diagnostic & Translational
    Sciences, Norfolk, VA 23529, USA
References
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Document Type
Publication order reference
Identifiers
YADDA identifier
bwmeta1.element.-psjd-doi-10_2478_nanome-2014-0002
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