Laminin- and basement membranepolycaprolactone blend nanofibers as a scaffold for regenerative medicine

• Authors: Rebekah A. Neal , Steven M. Lenz , Tiffany Wang , Daniel Abebayehu , Benjamin P.C. Brooks , Roy C. Ogle , Edward A. Botchwey
• Languages of publication: EN

Abstracts

EN

Mimicking one or more components of the
basement membrane (BM) holds great promise for
overcoming insufficiencies in tissue engineering
therapies. We have electrospun laminin nanofibers (NFs)
isolated from the murine Engelbreth-Holm Swarm (EHS)
tumor and evaluated them as a scaffold for embryonic
stem cell culture. Seeded human embryonic stem cells
were found to better maintain their undifferentiated,
colony environment when cultured on laminin NFs
compared to laminin mats, with 75% remaining
undifferentiated on NFs. Mouse embryonic stem cells
cultured on 10% laminin-polycaprolactone (PCL) NFs
maintained their colony formation for twice as long
without passage compared to those on PCL or gelatin
substrates. In addition, we have established a protocol
for electrospinning reconstituted basement membrane
aligned (RBM)-PCL NFs within 10° of angular deviation.
Neuron-like PC12 cells show significantly greater
attachment (p < 0.001) and percentage of neuriteextending
cells in vitro on 10% RBM-PCL NFs when
compared to 1% and 0% RBM-PCL NFs (p < 0.015 and p
< 0.001, respectively). Together, these results implicate
laminin- and RBM-PCL scaffolds as a promising
biomimetic substrate for regenerative medicine
applications.

Keywords

EN

basement membrane; laminin; polycaprolactone; embryonic stem cells; peripheral nerve regeneration

• Publisher: Versita
• Journal: Nanomaterials and the Environment
• Year: 2014
• Volume: 2
• Issue: 1

Dates

received

1 - 1 - 2014

online

1 - 9 - 2014

accepted

4 - 2 - 2014

Contributors

author

Rebekah A. Neal

• University of Virginia Department of Biomedical Engineering,
  Charlottesville, VA 22903, USA

author

Steven M. Lenz

• University of Virginia Department of Biomedical Engineering,
  Charlottesville, VA 22903, USA

author

Tiffany Wang

• Georgia Institute of Technology and Emory University
  Department of Biomedical Engineering, Atlanta, Georgia 30332, USA

author

Daniel Abebayehu

• University of Virginia Department of Biomedical Engineering,
  Charlottesville, VA 22903, USA

author

Benjamin P.C. Brooks

• University of Virginia Department of Biomedical Engineering,
  Charlottesville, VA 22903, USA

author

Roy C. Ogle

• Old Dominion University School of Medical Diagnostic
  & Translational Sciences, Norfolk, VA 23529, USA

author

Edward A. Botchwey

• Georgia Institute of Technology and Emory University
  Department of Biomedical Engineering, Atlanta, Georgia 30332, USA

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Identifiers

DOI

10.2478/nanome-2014-0001