Selective determination of penicillin G from tap water
and milk samples using surface molecularly imprinted
polymers as solid-phase extraction sorbent

• Authors: Wei Du , Huiyan Zhou , Zhimin Luo , Penglei Zheng , Pengqi Guo , Ruimiao Chang , Chun Chang , Qiang Fu
• Languages of publication: EN

Abstracts

EN

In this study, a selective sample preparation
method coupling surface molecularly imprinting polymers
(SMIPs) with solid-phase extraction (SPE) was developed
for the determination of penicillin G from tap water and
milk samples. SMIPs for penicillin G were synthesized
by using silica gel as supporting matrix, methacrylic
acid (MAA) as the functional monomer, ethylene glycol
dimethacrylate (EDMA) as the cross-linker, methanolacetonitrile
(1:1,v/v) as the solvents and 2,2′-azo-bisisobutyronitrile
(AIBN) as the initiator. Characterization
and adsorption experiments revealed that SMIPs
exhibited large adsorption capacity, high recognition
ability and high rate of mass transfer. Application of
SMIPs in SPE followed by HPLC was done to selectively
determine penicillin G from tap water and milk samples.
Under the optimal conditions, the proposed method
demonstrated high linearity with the concentration of
penicillin G ranging from 0.12 to 200 μg mL-1. The limits of
detection (LOD) in tap water and milk were 0.05, 0.03μg
mL-1, respectively. The limits of quantification (LOQ) in
tap water and milk were 0.25, 0.12 μg mL-1, respectively.
The recoveries of penicillin G at three spiked levels were
ranged from 78.9 to 85.5 % with intra-day and inter-day
relative standard deviation values below 4.8 %.

Keywords

EN

surface molecularly imprinted polymers; solidphaseextraction; silica gel; penicillin G

• Publisher: De Gruyter Open
• Journal: Molecular Imprinting
• Year: 2014
• Volume: 2
• Issue: 1

Dates

accepted

1 - 10 - 2014

online

21 - 10 - 2014

received

27 - 6 - 2014

Contributors

author

Wei Du

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Huiyan Zhou

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Zhimin Luo

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Penglei Zheng

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Pengqi Guo

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Ruimiao Chang

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Chun Chang

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

author

Qiang Fu

• School of Pharmacy, Xi’an Jiaotong
  University, Xi’an 710061, PR China

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Identifiers

DOI

10.2478/molim-2014-0002