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2014 | 2 | 1 |

Article title

Selective determination of penicillin G from tap water
and milk samples using surface molecularly imprinted
polymers as solid-phase extraction sorbent


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In this study, a selective sample preparation
method coupling surface molecularly imprinting polymers
(SMIPs) with solid-phase extraction (SPE) was developed
for the determination of penicillin G from tap water and
milk samples. SMIPs for penicillin G were synthesized
by using silica gel as supporting matrix, methacrylic
acid (MAA) as the functional monomer, ethylene glycol
dimethacrylate (EDMA) as the cross-linker, methanolacetonitrile
(1:1,v/v) as the solvents and 2,2′-azo-bisisobutyronitrile
(AIBN) as the initiator. Characterization
and adsorption experiments revealed that SMIPs
exhibited large adsorption capacity, high recognition
ability and high rate of mass transfer. Application of
SMIPs in SPE followed by HPLC was done to selectively
determine penicillin G from tap water and milk samples.
Under the optimal conditions, the proposed method
demonstrated high linearity with the concentration of
penicillin G ranging from 0.12 to 200 μg mL-1. The limits of
detection (LOD) in tap water and milk were 0.05, 0.03μg
mL-1, respectively. The limits of quantification (LOQ) in
tap water and milk were 0.25, 0.12 μg mL-1, respectively.
The recoveries of penicillin G at three spiked levels were
ranged from 78.9 to 85.5 % with intra-day and inter-day
relative standard deviation values below 4.8 %.







Physical description


1 - 10 - 2014
21 - 10 - 2014
27 - 6 - 2014


  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China
  • School of Pharmacy, Xi’an Jiaotong
    University, Xi’an 710061, PR China


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