The multi-stage 20-m shuttle run fitness test (20mMSFT) is a popular field test which is widely used to measure aerobic fitness by predicting maximum oxygen uptake (VO2 max) and performance. However, the velocity at which VO 2 max occurs (vVO 2 max) is a better indicator of performance than VO 2 max, and can be used to explain inter-individual differences in performance that VO 2 max cannot. It has been reported as a better predictor for running performance and it can be used to monitor athletes’ training for predicting optimal training intensity. This study investigated the validity and suitability of predicting VO2max and vVO2max of adult subjects on the basis of the performance of the 20mMST. Forty eight (25 male and 23 female) physical education students performed, in random order, a laboratory based continuous horizontal treadmill test to determine VO2max, vVO 2 max and a 20mMST, with an interval of 3 days between each test. The results revealed significant correlations between the number of shuttles in the 20mMSFT and directly determined VO 2 max (r = 0.87, p<0.05) and vVO 2 max (r = 0.93, p<0.05). The equation for prediction of VO 2 max was y = 0.0276x + 27.504, whereas for vVO 2 max it was y = 0.0937x + 6.890. It can be concluded that the 20mMSFT can accurately predict VO 2 max and vVO 2 max and this field test can provide useful information regarding aerobic fitness of adults. The predicted vVO 2 max can be used in monitoring athletes, especially in determining optimal training intensity.