The aim of this study was to assess the reliability of isokinetic and ISO knee extensor and flexor muscle strength when using the REV9000 (Technogym) isokinetic dynamometer. Moreover, the reliability of several strength imbalance indices and bilateral ratios were also examined. Twenty-four physically active healthy subjects (age 23±3 years) underwent three testing sessions, two on the same day and a third, 7 days later. All sessions proceeded in the same order: five concentric contractions at 60ºs-1 followed by an isometric contraction (5 seconds) and five eccentric contractions (60ºs-1). The results of this study showed a high reproducibility in eccentric (0.95-0.97), concentric (0.95- 0.96) and isometric (0.93-0.96), isokinetic strength for knee extensor and flexor muscles, thus indicating that the REV9000 isokinetic dynamometer can be used in future sports performance studies. A low-to-moderate reliability was found in the isokinetic strength bilateral ratios while the Hamstring:Quadricep concentric ratio showed moderate reliability. The highest reliability (>0.90) was observed in the dynamic control ratio (Hamstring eccentric:Quadricep concentric) which consequently confirms that it is a more valid indicator for imbalanced reciprocal parameters and can be used in rehabilitation and sports medicine.