rapid detection for the inhibition of phosphoglucose isomerase from Escherichia coli by mercury(II) chloride based on TLC-autographic analysis – preliminary studies

• Authors: Katarzyna Paradowska , Joanna Lutek , Grazyna Ginalska
• Languages of publication: EN

Abstracts

EN

The quest for new techniques for screening inhibitors of phosphoglucose isomerase is crucially important owing to therapeutic control of chronic bacterial infections associated with the biosynthesis of bacterial biofilm. According to the new method, yellowish zones against the purple background could be visually observed where phosphoglucose isomerase activity was inhibited. The new protocol with NADPH/NBT/PMS staining for TLC-autographic method was able to detect PGI inhibition by pure reference substance as mercury(II) chloride.

Keywords

EN

TLC-autography; phosphoglucose isomerase; inhibitor; E. coli ATCC 25922; biofilm

• Publisher: De Gruyter Open
• Journal: Current Issues in Pharmacy and Medical Sciences
• Year: 2014
• Volume: 27
• Issue: 2
• Pages: 127-130

Dates

published

1 - 6 - 2014

accepted

11 - 8 - 2014

received

21 - 2 - 2014

online

25 - 11 - 2014

Contributors

author

Katarzyna Paradowska

• Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland

author

Joanna Lutek

• Student of Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland

author

Grazyna Ginalska

• Chair and Department of Biochemistry and Biotechnology, Medical University of Lublin, Poland

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Identifiers

DOI

10.2478/cipms-2014-0030