In vitro evaluation of immunogenic properties of active dressings

• Authors: Joanna Orlowska , Urszula Kurczewska , Katarzyna Derwinska , Wojciech Orlowski , Daria Orszulak-Michalak
• Languages of publication: EN

Abstracts

EN

The aim of this study was in vitro evaluation of the level of the immune response in relation to wound dressings composed of alginate, calcium carboxymethylcellulose, and dibutyrylochitin and determination of the direction of response, which will make referring next to the results of in vivo phase possible. The subject of the experiments was to examine the commercially available, biodegradable alginate dressing, commercially available but not biodegradable dressing constructed from the sodium carboxymethylcellulose, and synthesized in house biodegradable dressing constructed of the dibutyrylchitin. To determine the direction of the immune response, the degree of secretion of pro-inflammatory interleukin (IL-1, IL-6) and antiinflammatory (IL-10) interleukin from murine fibroblasts having contact with the tested dressings (ELISA enzyme linked immunosorbent assay), was tested.

Keywords

EN

dressings; immune response; inflammation

• Publisher: De Gruyter Open
• Journal: Current Issues in Pharmacy and Medical Sciences
• Year: 2014
• Volume: 27
• Issue: 1
• Pages: 55-60

Dates

published

1 - 6 - 2014

accepted

10 - 3 - 2014

received

20 - 2 - 2014

online

30 - 5 - 2014

Contributors

author

Joanna Orlowska

• Ph.D. Student, Medical University of Lodz, Pl. Hallera 1, 90-647 Lodz, Poland
• Science and Research Laboratory Dermin Sp. z o.o., Milionowa 23, 93-193 Lodz, Po

author

Urszula Kurczewska

• Department of Biopharmaceutics, Medical University of Lodz, Muszynskiego 1, 90-153 Lodz, Poland

author

Katarzyna Derwinska

• Science and Research Laboratory Dermin Sp. z o.o., Milionowa 23, 93-193 Lodz, Po

author

Wojciech Orlowski

• Science and Research Laboratory Dermin Sp. z o.o., Milionowa 23, 93-193 Lodz, Po

author

Daria Orszulak-Michalak

• Department of Biopharmaceutics, Medical University of Lodz, Muszynskiego 1, 90-153 Lodz, Poland

References

• 1. Andersen T., et al.; Alginates as biomaterials in tissue engineering, Carbohydr. Chem., 37, 2012
• 2. Anderson J.M.; Biological responses to materials, Annu. Rev. Mater.Res. 31, 2001[Crossref]
• 3. Barnett S., Varley S.; The effects of calcium alginate on wound healing, Ann R Coll Surg Engl., 69, 4, 1987
• 4. Barrientos S., et al.; Growth factors and cytokines in wound healing, Wound Repair Regen. 16, 5, 2008[Crossref][WoS]
• 5. Burrow T., Welch M.J.; The development and use of alginate fibres in nonwovens for medical end-uses. In: Cusick GE, ed. Nonwoven Conference Papers, UMIST 1983
• 6. Eberlein T., et al; Comparison of PHMB-containing dressing and silver dressings in patients with critically colonised or locally infected wounds, J Wound Care, 21, 1, 2012.
• 7. Eming SA., et al.; Accelerated wound closure in mice deficient for interleukin-10, J.Pathol. 170.1, 2007.[WoS]
• 8. Gallin J. I., et al.; Inflammation: Basic Principles and Clinical Correlates, New York Raven, 1999 2nd ed.
• 9. Gallucci RM., et al; Interleukin-6 treatment augments cutaneous wound healing in immunosuppressed mice, J Interferon Cytokine Res., 21, 8, 2001.
• 10. Iannuccelli V., et al; Biodegradable intraoperative system for bone infection treatment II. In vivo evaluation. Int J Pharm, 143, 1996.
• 11. Ishikawa K et al; Self-setting barrier membrane for guided tissue regeneration method: initial evaluation of alginate membrane made with sodium alginate and calcium chloride aqueous solutions.J Biomed Mater Res., 47, 1999.[Crossref]
• 12. Jones V., Harding K.; Chronic Wound Care: A Clinical Source Book for Healthcare Professionals, 245-251, 2001
• 13. Kaneda K, Kuroda S, Goto N, Sato D, Ohya K, Kasugai S. ; Is sodium alginate an alternative haemostatic material in the tooth extraction socket?, J Oral Tissue Engin; 5, 2008
• 14. Krucinska I., et al.; Dibutyrylchitin nonwoven biomaterials manufactured directly from polymer solution. International Conference FiberMed06 - Fibrous Products in Medical and Health Care, Tampere, Finland, 2006
• 15. Krucińska I., et al.; Producing Wound Dressing Materials from Chitin Derivatives by Forming Nonwovens Directly from Polymer Solution, Fibres & Textiles in Eastern Europe, 15, 5-6, 2007
• 16. Krucińska I., et al..; Biological Estimation of Dibutyrylchitin Nonwovens Manufactured by the Spraying of Polymer Solution Technique, in Progress on Chemistry and Application of Chitin and its Derivative, Polish Chitin Society, Polish Chitin Society, Monograph XI, 2006
• 17. Krzyżanowska-Gołąb D., Lemańska-PerekA., Kątnik-Prastowska I.; Fibronektyna jako aktywny składnik macierzy pozakomórkowej, Postepy Hig Med. Dosw. (online), 61, 2007
• 18. Labler L .et.al.; Vacuum-assisted closure therapy increases local interleukin-8 and vascular endothelial growth factor levels in traumatic wounds, J Trauma., 66,3,2009.[WoS]
• 19. Mano J.et al.; Natural origin biodegradable systems in tissue engineering and regenerative medicine: present status and some moving trends, J R Soc. Interface. 22, 4, 2007[WoS]
• 20. Matthew IR.et al; Subperiosteal,behavior of alginate and cellulose wound dressing materials. Biomaterials, 16, 4, 1995[Crossref]
• 21. Muzzarelli R.A. et al.; The biocompatibility of dibutyrylchitin in the context of wound dressings. Biomaterials, 26, 29, 2005[Crossref]
• 22. Pielka S. et al.; Healing acceleration by a textile dressings containing dibutyrylchitin and chitin. Fibres & Textiles in Eastern Europe, 2, 41, 2003.
• 23. Pluta J., Haznar D.; Właściwości fizykochemiczne oraz kinetyka uwalniania substancji leczniczej z matryc żelatynowo-alginianowych, Polimery w Medycynie, 36, 3, 2006.
• 24. Schoukens G., Kiekens P., Krucińska I.; New bioactive textile dressing materials from dibutyrylchitin, Intern. J. of Clothing Science and Technology, 21, 2-3, 2009
• 25. Szewczyk M. et al.: Zasady wyboru opatrunków hydrofiber i alginianowych w leczeniu owrzodzeń żylnych, Zakażenia, 6, 2004
• 26. Szosland et al.; Opatrunki z dibutyrylochityny i chityny wspomagające gojenie się ran, IV Międzynarodowa Konferencja Naukowa Medtex 2002, Łódź.
• 27. Szosland L., Janowska G.; Patent PL No 169077 B1, 1996
• 28. Thomay A.A. et al.; Disruption of Interleukin-1 Signaling Improves the Quality of Wound Healing, J Pathol. 174, 6, 2009.[WoS]
• 29. Turner T. D.; The development of wound management products, Wounds, 3, 1989.
• 30. Vercellotti G. M. et al.; Inflamed Fibronectin: An Altered Fibronectin Enhances Neutrophil Adhesion, Blood, 62, 5, 1983
• 31. Wang L., et al.;Evaluation of sodium alginate for bone marrow cell tissue engineering, Biomaterials, 24, 20, 2003[Crossref]
• 32. Winter G.D.; Formation of the Scab and the Rate of Epithelization of Superficial Wounds in the Skin of the Young Domestic Pig, Nature, 193, 1962[Crossref]
• 33. Wu S. F. et al; Repair of facial nerve with alginate sponge without suturing: an experimental study in cats. Scand J Plast. Reconstr. Surg. Hand Surg., 36, 3, 2002.

Identifiers

DOI

10.2478/cipms-2014-0014