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2014 | 1 | 1 |

Article title

Biosynthesis of ethyl butyrate with immobilized
Candida rugosa lipase onto modified Eupergit®C


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Physical description


22 - 6 - 2014
26 - 8 - 2014
3 - 10 - 2014


  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy
  • Department of
    Biotechnology, Chemistry and Pharmacy, University of Siena, Via A.
    Moro 2, Siena, Italy


  • [1] Schwab W., Davidovich-Rikanati R., Lewinsohn E., Biosynthesisof plant-derived flavor compounds, Plant J., 2008, 54, 712-732.[WoS][Crossref]
  • [2] Rajendran A., Palanisamy A., Thangavelu V., Lipase catalyzedester synthesis for food processing industries, Braz. Arch. Biol.Technol., 2009, 52, 207–219.[Crossref][WoS]
  • [3] Jin Z., Ntwali J., Han S.-Y., Zheng S.-P., Lin Y., Production offlavor esters catalyzed by CALB-displaying Pichia pastoriswhole-cells in a batch reactor, J. Biotechnol., 2012, 159,108–114.[WoS]
  • [4] Mahapatra P., Kumari A., Garlapati V.K., Banerjee R, Nag A.,Enzymatic synthesis of fruit flavor esters by immobilized lipasefrom Rhizopus oligosporus optimized with response surfacemethodology, J. Mol. Catal. B: Enzymatic, 2009, 60, 57–63.[Crossref][WoS]
  • [5] Shu C., Cai J., Huang L., Zhu X., Xu Z., Biocatalytic productionof ethyl butyrate from butyric acid with immobilized Candidarugosa lipase on cotton cloth, J. Mol. Catal. B: Enzymatic, 2011,72, 139–144.[Crossref]
  • [6] Pires-Cabral P., da Fonseca M.M.R., Ferreira-Dias S., Esterificationactivity and operational stability of Candida rugosa lipase immobilized in polyurethane foams in the production ofethyl butyrate, Biochem. Eng. J., 2010, 48, 246–252.[WoS][Crossref]
  • [7] Pires-Cabral P., da Fonseca M.M.R., Ferreira-Dias S., Modellingthe production of ethyl butyrate catalysed by Candida rugosalipase immobilised in polyurethane foams, Biochem. Eng. J.,2007, 33, 148–158.[Crossref][WoS]
  • [8] Berger R.G., Biotechnology of flavours-the next generation,Biotechnol. Lett., 2009, 31, 1651–1659.[Crossref][WoS]
  • [9] Schrader J., Etschmann M.M.W., Sell D., Hilmer J.M.,Rabenhorst J., Applied biocatalysis for the synthesis of naturalflavour compounds - current industrial processes and futureprospects, Biotechnol. Lett., 2004, 26, 463–472.[Crossref]
  • [10] Serra S., Fuganti C., Brenna E., Biocatalytic preparation ofnatural flavours and fragrances, Trends Biotechnol., 2005, 23,193–198.[Crossref]
  • [11] Kapoor M., Gupta M.N., Lipase promiscuity and its biochemicalapplications, Process Biochem., 2012, 47, 555-569.[WoS][Crossref]
  • [12] Zhang B., Weng Y., Xu H., Mao Z., Enzyme immobilization forbiodiesel production, Appl. Microbiol. Biotechnol. 2012, 93,61–70.[Crossref]
  • [13] Varma M.N., Madras G., Kinetics of synthesis of butyl butyrateby esterification and transesterification in supercritical carbondioxide, J. Chem. Technol. Biotechnol., 2008, 83, 1135–1144.[Crossref][WoS]
  • [14] Krishna S.H., Divakar S., Prapulla S.G., Karanth N.G., Enzymaticsynthesis of isoamyl acetate using immobilized lipase fromRhizomucor miehei, J. Biotechnol., 2001, 87, 193-201.
  • [15] Razafindralambo H., Blecker C., Lognay G., Marlier M.,Wathelet P., Severin M., Improvement of enzymatic synthesisconversions of flavour acetates: the example of the isoamylacetate, Biotechnol. Lett., 1994,16, 247-50.[Crossref]
  • [16] Song J., Kahveci D., Chen M., Guo Z., Xie E., Xu X., BesenbacherF., Dong M., Enhanced Catalytic Activity of Lipase Encapsulatedin PCL Nanofibers, Langmuir, 2012, 28, 6157−6162.[Crossref][WoS]
  • [17] Thakar A., Madamwar D., Enhanced ethyl butyrate productionby surfactant coated lipase immobilized on silica, ProcessBiochem., 2005, 40, 3263–3266.[Crossref]
  • [18] Bayramoglu G., Kaya B., Arica M.Y., Immobilization of Candidarugosa lipase onto spacer-arm attached poly(GMAHEMAEGDMA)microspheres, Food Chemistry, 2005, 92, 261–268.[Crossref]
  • [19] Kim M.I., Ham H.O., Oh S.-D., Park H.G., Chang H.N., Choi S.-H.,Immobilization of Mucor javanicus lipase on effectively functionalizedsilica nanoparticles, J. Mol. Catal. B: Enzymatic, 2006,39, 62–68.[Crossref]
  • [20] Katchalski-Katsir E., Kraemer D., Eupergit C, a carrier forimmobilization of enzymes of industrial enzymes, J. Mol. Catal.B: Enzymatic, 2000, 10, 157-176.[Crossref]
  • [21] Aguila S., Vazquez-Duhalt R., Covarrubias C., Pecchi G.Alderete J.B., Enhancing oxidation activity and stability ofiso-1-cytochrome c and chloroperoxidase by immobilization innanostructured supports, J. Mol. Cat. B: Enzymatic, 2011, 70,81-87.
  • [22] Yahya A.R.M., Anderson W.A., Moo-Young M.E., Ester synthesisin lipase-catalyzed reactions, Enzyme Microb. Technol., 1998,23, 438-50.[Crossref]
  • [23] De B.K., Chatterjee T., Bhattacharyya D.K., Synthesis ofgeranyl and citronellyl esters of coconut oil fatty acids throughalcoholysis by Rhizomucor miehei lipase catalysis, J. Am. Oil.Chem. Soc., 1999, 76, 1501-4.[Crossref]
  • [24] Chowdary G.V., Ramesh M.N., Prapulla S.G., Enzymaticsynthesis of isoamyl isovalerate using immobilized lipase fromRhizomucor miehei: a multivariate analysis, Process Biochem.,2000, 36, 331-9.[Crossref]
  • [25] Sun S.W., Lin Y.C., Weng Y.M., et al., Efficiency improvementson ninhydrin method for amino acid quantification, J. FoodComp. Anal., 2006, 19, 112-117.[Crossref]
  • [26] Ruiz, C., Pastor, F. I. J., Diaz, P., Analysis of Bacillus megateriumlipolytic system and cloning of LiA, a novel subfamily I.4bacterial lipase, FEMS Microbiol. Lett., 2002, 217, 263-267.
  • [27] Bulov L., Mosbach K., The expression in E. coli of a polymericgene coding for an esterase mimic catalyzing the hydrolysis ofp-nitrophenyl esters, FEBS Lett., 1987, 210, 147-152.
  • [28] Prim N., Blanco A., Martinez J., Pastor F.I.J., Diaz P., EstA, agene coding for a cell-bound esterase from Paenibacillus sp.BP-23, is a new member of the bacterial subclass of type Bcarboxylesterases, Res. Microbiol., 2000, 151, 303-312.
  • [29] Knezevic Z., Milosavic N., Bezbradica D., Jakovljevic Z.,Prodanovic R., Immobilization of lipase from Candida rugosaon Eupergit C supports by covalent attachment, Biochem.Engin. J., 2006, 30, 269-278.
  • [30] Li H., Zhang X., Characterization of thermostable lipase fromthermophilic Geobacillus sp. TW1, Protein Expr. Purif., 2005,42, 153–159.
  • [31] Hirsh S.L., Bilek M.M., Nosworthy N.J., Kondyurin A., dosRemedios C.G., McKenzie D.R., A comparison of covalentimmobilization and physical adsorption of a cellulase enzymemixture, Langmuir, 2010, 26, 14380–14388.[WoS][Crossref]
  • [32] Garcia A., Oh S., Engler C.R., Cellulase immobilization on Fe3O4and characterization, Biotechnol. Bioeng., 1989, 33, 321–326.[Crossref]
  • [33] Lorenzoni A.S.G., Graebin N.G.,Martins A.B., Fernandez-Lafuente R., Ayub M.A.Z. Rodrigues R.C., Optimization ofpineapple flavour synthesis by esterification catalysed byimmobilized lipase from Rhizomucor miehei, Flavour Fragr. J.,2012, 27, 196–200.
  • [34] Spinelli D., Fatarella E., Di Michele A., Pogni R., Immobilizationof fungal (Trametes versicolor) laccase onto Amberlite IR-120 Hbeads: Optimization and characterization, Process Biochem.,2013, 48, 218–223.[WoS][Crossref]
  • [35] Fatarella E., Spinelli D., Ruzzante M., Pogni R., Nylon 6 film andnanofiber carriers: Preparation and laccase immobilizationperformance, J. Mol. Catal. B: Enzymatic, 2014, 102, 41–47.[WoS]
  • [36] Hills G., Industrial use of lipases to produce fatty acid Esters,Eur. J. Lipid Sci. Technol., 2003, 105, 601–607.[Crossref]
  • [37] Laane C., Boeren S., Vos K., Veeger C., Rules for optimization ofbiocatalysis in organic solvents, Biotechnol. Bioeng., 1987, 30,81-7.[Crossref]
  • [38] Carta G., Gainer J.L., Gibson M.E., Synthesis of esters using anylon-immobilized lipase in batch and continuous reactors,Enzyme Microb. Tech., 1992, 14, 904-910.[Crossref]
  • [39] Martins A.B., da Silva A.M., Scheina M.F., Garcia-Galan C., AyubM.A.Z., Fernandez-Lafuente R., Rodrigues R.C., Comparisonof the performance of commercial immobilized lipases in thesynthesis of different flavor esters, J. Mol. Catal. B: Enzymatic,2014, 105, 18–25.[WoS][Crossref]
  • [40] Syamsul K.M.W., Salina M.R., Siti S.O., Hanina M.N.,Basyaruddin M.A.R., Jusoff K., Green Synthesis of LaurylPalmitate via Lipase-Catalyzed Reaction, World App. Sci. J.,2010, 11,401-407.
  • [41] Kumar A. Kanwar S.S., Synthesis of isopropyl ferulate usingsilica-immobilized lipase in an organic medium, Enzyme Res.,2011, Article ID 718949.[Crossref]
  • [42] Sharma S. Kanwar S.S., Organic Solvent Tolerant Lipases andApplications, The Scientific World Journal, vol. 2014, Article ID625258, 15 pages, 2014. doi:10.1155/2014/625258.[Crossref]
  • [43] Cesarini S., Pastor J., Diaz P., Improvement of P. aeruginosa42A2 lipase preparations for FAMEs production, both in immobilized and soluble form, J. Mol. Catal. B: Enzymatic,2014, 99, 1– 7.[Crossref][WoS]

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