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2004 | 2 | 3 | 417-424
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Determination of aristolochic acid by capillary zone electrophoresis

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A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.
Physical description
1 - 9 - 2004
1 - 9 - 2004
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • [1] G.M. Lord, T. Cook, V.M. Arlt, H.H. Schmeiser, G. Williams and C.D. Pusey: “Urothelial malignant disease and Chinese herbal nephropathy”, Lancet, Vol. 358, (2001), pp. 1515–1516.[Crossref]
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  • [8] E.S. Ong and S.O. Woo: “Determination of aristolochic acids in medicinal plants (Chinese) prepared medicine using capillary zone electrophoresis”, Electrophoresis, Vol. 22, (2001) pp. 2236–2241.<2236::AID-ELPS2236>3.0.CO;2-T[Crossref]
  • [9] H.A. Priestap, S.L. Iglesias, M.F. Desimone and L.E. Diaz: “Determination of aristolochic acids by capillary electrophoresis”, J. Capillary Electrop., Vol. 8, (2003), pp. 39–43.
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