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2004 | 2 | 3 | 417-424

Article title

Determination of aristolochic acid by capillary zone electrophoresis


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A simple and rapid capillary zone electrophoresis (CZE) method for the determination of aristolochic acid (AA) in dietary supplements and selected herbs is described. A clear separation of AA from other sample constituents was achieved within 5 minutes without any sample clean up. A mixture of 20 mM-morpholinethanesulphonic acid+10 mM-BisTrisPropane+0.2% hydroxyethylcelullose in 10% methanol serves as a background electrolyte. The linearity, accuracy, intra-assay and detection limit of the developed method are 200–6000 ng/mL, 95–103%, 3.5%, and 50 ng/ml, respectively. Ease of use, sufficient sensitivity and low running cost are the most important attributes of the CZE method. The proposed CZE method was compared with HPLC.










Physical description


1 - 9 - 2004
1 - 9 - 2004


  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic
  • Institute of Chemical Technology, Technicka 1905/5, 166 28, Prague 6, Czech Republic


  • [1] G.M. Lord, T. Cook, V.M. Arlt, H.H. Schmeiser, G. Williams and C.D. Pusey: “Urothelial malignant disease and Chinese herbal nephropathy”, Lancet, Vol. 358, (2001), pp. 1515–1516. http://dx.doi.org/10.1016/S0140-6736(01)06576-X[Crossref]
  • [2] J.P. Cosyns: “Aristolochic acid and ‘Chinese herbs nephropathy’-A review of the evidence to date”, Drug Safety, Vol. 26, (2003) pp. 33–48. http://dx.doi.org/10.2165/00002018-200326010-00004[Crossref]
  • [3] J.R. Ioset, G.E. Raoelison and K. Hostettmann: “Detection of aristolochic acid in Chinese phytomedicines and dietary supplements used as slimming regimens”, Food Chem. Toxicol., Vol. 41, (2003) pp. 29–36. http://dx.doi.org/10.1016/S0278-6915(02)00219-3[Crossref]
  • [4] S. Zhou, H. Koh, Y. Gao, Z. Gong and E.J.D Lee: “Herbal bioactivation: the good, the bad and the ugly”, Life Sci., Vol. 74, (2004), pp. 935–968. http://dx.doi.org/10.1016/j.lfs.2003.09.035[Crossref]
  • [5] J.H. Jon, S.S. Chen and T.S. Wu: “Facile reversed-phase HPLC resolution and quantitative determination of aristolochic acid and aristolactam analogues in traditional Chinese medicine”, J. Liq. Chromatogr. R T., Vol. 26, (2003) pp. 3067–3078.
  • [6] J.R. Ioset, G.E. Raoelison and K. Hostettmann: “An LC/DAD-UV/MS method for the rapid detection of aristolochic acid in plant preparations”, Planta Med., Vol. 68, (2002), pp. 856–858. http://dx.doi.org/10.1055/s-2002-34413[Crossref]
  • [7] T.Y. Lee, M.L. Wu, J.F. Deng and D.F. Hwang: “High-performance liquid chromatographic determination for aristolochic acid in medicinal plants and slimming products”, J. Chromatogr., Vol. 766, (2001), pp. 169–174.
  • [8] E.S. Ong and S.O. Woo: “Determination of aristolochic acids in medicinal plants (Chinese) prepared medicine using capillary zone electrophoresis”, Electrophoresis, Vol. 22, (2001) pp. 2236–2241. http://dx.doi.org/10.1002/1522-2683(20017)22:11<2236::AID-ELPS2236>3.0.CO;2-T[Crossref]
  • [9] H.A. Priestap, S.L. Iglesias, M.F. Desimone and L.E. Diaz: “Determination of aristolochic acids by capillary electrophoresis”, J. Capillary Electrop., Vol. 8, (2003), pp. 39–43.
  • [10] Z. Sun, L. Liu, X. Zheng, Ch. Fan, Q. Wang and G. Li: “An easy and rapid method to determine aristolochic acids I and II with high sensitivity”, Anal. Bioanal. Chem., Vol. 378, (2004) pp. 388–390. http://dx.doi.org/10.1007/s00216-003-2209-2[Crossref]

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