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2004 | 2 | 1 | 82-90

Article title

Determination of inorganic arsenic species As(III) and As(V) by high performance liquid chromatography with hydride generation atomic absorption spectrometry detection


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The paper presents the principles and advantages of a technique combining high performance liquid chromatography and hydride generation atomic absorption spectrometry (HPLC-HGAAS) applied to speciation analysis of inorganic species of arsenic As(III) and As(V) in ground water samples. With separation of the arsenic species on an ion-exchange column in the chromatographic system and their detection by the hydride generation atomic absorption spectrometry, the separation of the analytical signals of the arsenic species was excellent at the limits of determination of 1.5 ng/ml As(III) and 2.2 ng/ml As(V) and RSD of 4.3% and 7.8% for the concentration of 25 ng/ml. The hyphenated technique has been applied for determination of arsenic in polluted ground water in the course of the study on migration of micropollutants. For total arsenic concentration two independent methods: HGICP-OES and HGAAS were used for comparison of results of real samples analysis.










Physical description


1 - 3 - 2004
1 - 3 - 2004


  • Department of Water and Soil Analysis, Adam Mickiewicz University, 24 Drzymały Street, 60-613, Poznań, Poland
  • Department of Hydrogeology and Waters Protection, Adam Mickiewicz University, 16 Maków Polnych Street, 61-686, Poznań, Poland
  • Laboratory of Atomic Spectrochemistry, Masaryk University, 2 Kotlářská Street, 611 37, Brno, Czech Republic


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  • [2] P. Niedzielski, M. Siepak, J. Przybyłek and J. Siepak: “Arsenic, antimony and selenium in water city of Poznań”, Betagraf, Poznań, 2002, (in Polish).
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  • [6] T. Nakazato, T. Taniguchi, H. Tao, M. Tominaga and A. Miyazaki: “Ion-exclusion chromatography combined with ICP-MS and hybride generation-ICP-MS for the determination of arsenic species in biological matrices”, J. Anal. At. Spectrom., Vol. 15, (2000), pp. 1546–1552. http://dx.doi.org/10.1039/b005981l[Crossref]
  • [7] Y. Bohari, A. Astruc, M. Astruc and J. Cloud: “Improvements of hybride generation of the speciation of arsenic in natural freshwater samples by HPLC-HG-AFS”, J. Anal. At. Spectrom., Vol. 16, (2001), pp. 774–778. http://dx.doi.org/10.1039/b101591p[Crossref]
  • [8] B. He, G. Jiang and X. Xu: “Arsenic speciation based on ion exchange high-performance liquid chromatography hyphenated with hydride generation atomic fluorescence and on-line UV photo oxidation”, Fresenius J. Anal. Chem., Vol. 368, (2000), pp. 803–808. http://dx.doi.org/10.1007/s002160000597[Crossref]
  • [9] J.T. Elteren, V. Stibilij and Z. Slejkovec: “Speciation of inorganic arsenic in some bottled Slovene mineral waters using HPLC-HGAFS and selective coprecipitation combined with FI-HGAFS”, Water Research, Vol. 36, (2002), pp. 2967–2974. http://dx.doi.org/10.1016/S0043-1354(01)00527-9[Crossref]
  • [10] D.L. Tsalev, M. Sperling and B. Welz: “Flow-injection hydride generation atomic absorption spectrometric study of the automated on-line pre-reduction of arsenate, methylarsonate and dimethylarsinate and high-performance liquid chromatographic separation of their L-cysteine complexes”, Talanta, Vol. 51, (2000), pp. 1059–1068. http://dx.doi.org/10.1016/S0039-9140(00)00297-6[Crossref]
  • [11] D.L. Tsalev, M. Sperling and B. Welz: “On-line UV-photooxidation with peroxodisulfate forautomated flow injection and for high-performanceliquid chromatography coupled to hydride generationatomic absorption spectrometry”, Spectrochimica Acta Part B, Vol. 55, (2000), pp. 3390–353.
  • [12] M.C. Villa-Lojo, E. Alonso-Rodriguez, P. Lopez-Mahia, S. Muniategui-Lorenzo and D. Prada-Rodriguez: “Coupled high performance liquid chromatography-microwave digestion-hydride generation-atomic absorption spectrometry for inorganic and organic arsenic speciation in fish tissue”, Talanta, Vol. 57, (2002), pp. 741–750. http://dx.doi.org/10.1016/S0039-9140(02)00094-2[Crossref]
  • [13] W.Ch. Tseng, M.H. Yang, T.P. Chen and Y.L. Huang: “Automated, continuous, and dynamic speciation of urinary arsenic in the bladder of living organisms using microdialysis sampling coupled on-line with high performance liquid chromatography and hydride generation atomic absorption spectrometry”, Analyst, Vol. 127, (2002), pp. 560–564. http://dx.doi.org/10.1039/b110053j[Crossref]
  • [14] Q. Xie, R. Kerrich, E. Irving, K. Liber and F. Abou-Shakra: “Determination of five arsenic species in aqueous samples by HPLC coupled with a hexapole collision cell ICP-MS”, J. Anal. At. Spectrom., Vol. 17, (2002), pp. 1037–1041. http://dx.doi.org/10.1039/b202172b[Crossref]
  • [15] G. Koellensperger, J. Nurmi, S. Hann, G. Stingeder, W.J. Fitz and W.W. Wenzel: “CE-ICP-SFMS and HPIC-ICP-SFMS for arsenic speciation in soil solution and soil water extracts”, J. Anal. At. Spectrom., Vol. 17, (2002), pp. 1042–1047. http://dx.doi.org/10.1039/b202875c[Crossref]
  • [16] P. Niedzielski, J. Siepak and M. Siepak: “Total Content of Arsenic, Antimony and Selenium in Groundwater Samples from Western Poland”, Pol. J. Environ. Stud., Vol. 5, (2001), pp. 347–350.

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