A kinetic method for para-nitrophenol determination based on its inhibitory effect on the catalatic reaction of catalase

• Authors: Claudia Mureşanu , Lucian Copolovici , Florina Pogącean
• Languages of publication: EN

Abstracts

EN

The inhibitory effect of para-nitrophenol on the catalytic reaction of catalase was investigated. Michaelis-Menten kinetic parameters were determined from Lineweaver-Burk plots obtained in the absence or in the presence of the inhibitor. The inhibitor pattern, revealed by the Lineweaver-Burk plots, suggested a fully mixed inhibition mechanism. Spectrophotometric monitoring of the indicator reaction:
$$H_2 O_2 \xrightarrow{{catalase,para - nitrophenol}}H_2 O + \tfrac{1}{2}O_2 $$
in conjunction with initial rate measurements was employed for the kinetic determination of the inhibitor. Calibration plots of initial rate vs. para-nitrophenol concentration were linear in the concentration range 0.9·10−5–2.5·10−5 mol/L and the detection limit was 3·10−6 mol/L (417 μg/L) para-nitrophenol. Interferences from other phenolic compounds like orto-cresole, meta-and orto-nitrophenol were observed.

Keywords

EN

Kinetic method; catalase; para-nitrophenol; hydrogen peroxide; inhibition; water analysis

• Publisher: De Gruyter Open
• Journal: Open Chemistry
• Year: 2005
• Volume: 3
• Issue: 4
• Pages: 592-604

Dates

published

1 - 12 - 2005

online

1 - 12 - 2005

Contributors

author

Claudia Mureşanu

• Facully of Chemistry and Chemical Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 400028, Cluj-Napoca, Romania

author

Lucian Copolovici

• Facully of Chemistry and Chemical Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 400028, Cluj-Napoca, Romania

author

Florina Pogącean

• Facully of Chemistry and Chemical Engineering, Babeş-Bolyai University, 11 Arany Janos Street, 400028, Cluj-Napoca, Romania

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Identifiers

DOI

10.2478/BF02475190