Ultrasound-assisted Matrix Solid Phase
Dispersion for the HPLC-DAD analysis of
amphenicols in shrimps

• Authors: Victoria F. Samanidou , Evgenia A. Makrygianni
• Languages of publication: EN

Abstracts

EN

Ultrasound-assisted matrix solid phase
dispersive extraction was applied for the selective
isolation and clean-up of three amphenicol antibiotics,
chloramphenicol (CAP), thiamphenicol (TAP) and
florfenicol (FFC) from shrimp. The target antibiotics
were separated on a LiChroCART-LiChrospher® 100
RP-18 (5 μm, 250 × 4 mm) analytical column in less than
9 min, with isocratic elution using a mixture of 70%
ammonium acetate (0.05 M) and 30% acetonitrile (v/v).
Matrix Solid Phase Dispersion protocol was optimized
in terms of extraction sorbent and elution solvent. Two
polymer based (Oasis and Nexus) sorbents and one
silica based (Lichrolut C18) were compared and different
elution solvents such as methanol, acetone, acetonitrile
and isopropanol were evaluated based on the achieved
recovery rates as well as on the cleanup efficiency. The
extraction procedure was performed with and without
sonication to evaluate the impact of ultrasounds.
TAP and FFC were monitored at 234 nm and CAP at 280 nm
by a photodiode array detector. The method was validated
according to the European Union Decision 2002/657/EC in
terms of linearity, selectivity, stability, accuracy, precision
and sensitivity. Detection capability values (CCb) were
64.6 μg/kg for TAP and 1046.8 μg/kg for FFC and 63.8 μg/
kg for CAP.

Keywords

EN

HPLC; amphenicols; shrimps; ultrasoundassisted MSPD; 657/2002/EC

• Publisher: De Gruyter Open
• Journal: Sample Preparation
• Year: 2014
• Volume: 2
• Issue: 1

Dates

online

29 - 5 - 2015

accepted

6 - 5 - 2015

received

9 - 3 - 2015

Contributors

author

Victoria F. Samanidou

• Laboratory of Analytical Chemistry, Department
  of Chemistry, Aristotle University of Thessaloniki, GR-54124,
  Thessaloniki, Greece

author

Evgenia A. Makrygianni

• Laboratory of Analytical Chemistry, Department
  of Chemistry, Aristotle University of Thessaloniki, GR-54124,
  Thessaloniki, Greece

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Identifiers

DOI

10.1515/sampre-2015-0003