Effect of pre-stain methanol washing
on the sensitivity of CBBR-250 staining
in SDS-polyacrylamide gels

• Authors: Rashid Saleem , Absar-ul Hasnain , Riaz Ahmad
• Languages of publication: EN

Abstracts

EN

Coomassie Brilliant Blue R-250 (CBBR-250) is
the most frequently used dye stain in protein research.
However, relatively poor sensitivity and long application
durations are the major limitations of this stain. In the
present study, we have investigated the effect of pre-stain
methanol washing on the sensitivity of the conventional
CBB staining method. Concentrations of methanol ranging
from 5 to 30% were prepared in 5% acetic acid. Pre-stain
washing of SDS-gels using acetic acid (5%) and methanol
were assessed at various timings and temperatures. Our
results demonstrate that pre-stain washing by methanol
greatly improves the sensitivity of the CBB stain and
facilitates the visualization of low molecular weight
components present in a complex muscle protein,
actomyosin. The outcome of this study also revealed
duration of pre-stain washing and temperature as other
factors that determine the sensitivity of CBB staining
method. Therefore, it is suggested that pre-stain washing
with methanol may improve the sensitivity of CBB stain in
SDS-PA gels.

Keywords

EN

Actomyosin; CBB staining; Gel electrophoresis; Methanol washing

• Publisher: De Gruyter Open
• Journal: Sample Preparation
• Year: 2014
• Volume: 2
• Issue: 1

Dates

accepted

26 - 2 - 2015

online

6 - 7 - 2015

received

9 - 12 - 2014

Contributors

author

Rashid Saleem

• Biochemical and Clinical Genetics
  Laboratory, Section of Genetics, Department of Zoology, Aligarh
  Muslim University, Aligarh-202002

author

Absar-ul Hasnain

• Biochemical and Clinical Genetics
  Laboratory, Section of Genetics, Department of Zoology, Aligarh
  Muslim University, Aligarh-202002

author

Riaz Ahmad

• Biochemical and Clinical Genetics
  Laboratory, Section of Genetics, Department of Zoology, Aligarh
  Muslim University, Aligarh-202002

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Identifiers

DOI

10.1515/sampre-2015-0001