Immunomodelling Characteristics of Mature Dendritic Cells Stimulated by Colon Cancer Cells Lysates

• Authors: Sebastian Radej , Jacek Roliński , Karol Rawicz-Pruszyński , Paweł Bury , Grzegorz Borowski , Jacek Furmaga , Andrzej Chrościcki , Grzegorz Wallner , Ryszard Maciejewski
• Languages of publication: EN

Abstracts

EN

Application of cells with high TAA (tumor associated antigen) presentation potential seems to be crucial in neoplasia immunotherapy. Such feature is distributed in dendritic cells, which present peptides from processed TAA - MHC molecules complex to the T cells of a host. The aim of the study was to assess the influence of colon neoplasia tissue lysate on functioning of generated autologous DC’s in the field of autologous CD4+ lymphocytes immunological response towards Th1/Th2 under in vitro environment together with comparison and assessment of DCs’ immunosuppressive properties acquired from patients with colon cancer. Material and methods. The population of this study consisted of 16 healthy- controls, 36colon cancer patients. Blood samples were collected 24h before planned surgery and preventive antibiotic therapy. Neoplastic tissue sample, was digested for cell lysates preparation. DC’s generation from PBMC was carried out in standard conditionsand medium enriched with rhGM-CSF and rhIL-4. Mature DC`s and cocultured autologous CD4 lymphocytes immunophenotype assessment was analyzed with flow cytometer. Intracellular and culture medium cytokines concentration was analyzed with ELISA and FACS method. Results. DC`s generated from colon cancer patients stimulated with lysates presented greater maturity, lower expression of CD206 antigen, significantly higher expression of HLA-DR, CD208 and CD209 and high intracellular expression of IL-12, compared to non-stimulated cells. Conclusions. The neoplastic tissue in vivo produces a number of substances having an unfavorable effect on immune system, our results suggests using lysates as good dendritic cells stimulators that possibly could have application in colon cancer immunotherapy

Keywords

EN

dendritic cells; colon cancer; cancer cells lysates; Th1; Th2

• Publisher: De Gruyter Open
• Journal: Polish Journal of Surgery
• Year: 2015
• Volume: 87
• Issue: 2
• Pages: 71-82

Dates

published

1 - 2 - 2015

online

3 - 7 - 2015

received

31 - 1 - 2015

Contributors

author

Sebastian Radej

• Department of Human Anatomy, Medical University in Lublin1 Kierownik: prof. dr hab. R. Maciejewski

author

Jacek Roliński

• Department of Clinical Immunology, Medical University in Lublin2 Kierownik: prof. dr hab. J. Roliński PhD student

author

Karol Rawicz-Pruszyński

• Department of Surgical Oncology, Medical University in Lublin

author

Paweł Bury

• Department of General and Gastroenterological Surgery and Surgical Oncology of the Alimentary Tract, Medical University in Lublin

author

Grzegorz Borowski

• Department of General and Gastroenterological Surgery and Surgical Oncology of the Alimentary Tract, Medical University in Lublin

author

Jacek Furmaga

• Department of General and Transplant Surgery and Nutritional Treatment, Medical University in Lublin

author

Andrzej Chrościcki

• Department of General and Oncological Surgery, District Specialist Hospital in Lublin

author

Grzegorz Wallner

• Department of General and Gastroenterological Surgery and Surgical Oncology of the Alimentary Tract, Medical University in Lublin

author

Ryszard Maciejewski

• Department of Human Anatomy, Medical University in Lublin1 Kierownik: prof. dr hab. R. Maciejewski

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Identifiers

DOI

10.1515/pjs-2015-0022