Preferences help
enabled [disable] Abstract
Number of results
2015 | 2 | 1 |
Article title

Cysteine-containing peptides are produced by
sequential clipping, but not released, from lupin
11S storage globulin during early germination

Title variants
Languages of publication
The digestion of the seed storage proteins is a
finely regulated process operated by several proteases
whose action is influenced by the exposure of specific
regions, which became progressively available upon
their action. We focused our study on the initial stages
of germination, where more subtle modifications to
the storage proteins are expected. Small-size peptides
containing cysteine residues and other possible metalbinding
regions are de facto produced but are not released
from the “parental” protein since they remain bound
trough disulphide bridges. The meaning of these findings
is discussed.
Physical description
20 - 1 - 2016
23 - 8 - 2015
30 - 10 - 2015
  • [1] Muentz K., Intracellular protein sorting and the formation ofprotein reserves in storage tissue cells of plant seeds. Bioch.Physiol. Pflanzen, 1989, 185, 315-335.
  • [2] Shutov A.D., Vaintraub I.A., Degradation of storage proteins ingerminating seeds. Phytochemistry, 1987, 26, 1557-1566.
  • [3] Shutov A.D., Baumlein H., Blattner F.R., Muentz K., Storage andmobilization as antagonistic functional constraints on seedstorage globulin evolution J. Exp. Bot., 2003, 54, 1645-1654.
  • [4] Duranti M., Consonni A., Magni C., Sessa F., Scarafoni A., Themajor proteins of lupin seed: characterisation and molecularproperties for use as functional and nutraceutical ingredients.Trends Food Sci. Technol., 2008, 19, 624–633.[WoS]
  • [5] Scarafoni A., Ronchi A., Prinsi B., Espen L., Assante G.,Venturini G., Duranti M., The proteome of exudates fromgerminating Lupinus albus seeds is secreted through aselective dual-step process and contains proteins involved inplant defense, FEBS J., 2013, 280, 1443-1459.[WoS]
  • [6] Bewley J.D., Seed germination and dormancy, Plant Cell, 1997,9, 1055-1066.
  • [7] Capraro J., Sessa F., Magni C., Scarafoni A., Maffioli E.,Tedeschi, G., Duranti, M., Proteolytic cleavage at twin arginineresidues affects structural and functional transitions of lupinseed 11S storage globulin, PLoS ONE (2015), 10(2): e0117406.doi: 10.1371/journal.pone.0117406.
  • [8] Bradford M.M., A rapid and sensitive method for thequantitation of micrograms quantities of proteins utilising theprinciple of protein-dye binding, Anal. Biochem., 1976, 72,248-254.
  • [9] Iametti S., De Gregori B., Vecchio G., Bonomi F., Modificationsoccur at different structural levels during the heat denaturationof β-lactoglobulin, Eur. J. Biochem., 1996, 237, 106-112.
  • [10] Laemmli U.K., Cleavage of structural proteins during theassembly of the head of bacteriophage T4, Nature, 1970, 227,660-665.
  • [11] Jin L.-T., Hwang S.-Y., Yoo G.-S., Choi, J.-K., A mass spectrometrycompatible silver staining method for protein incorporating anew silver sensitizer in sodium dodecyl sulfate-polyacrylamideelectrophoresis gels., Proteomics, 2006, 6, 2334–2337.
  • [12] Ellman G.L., A colorimetric method for determining low concentrationsof mercaptans, Arch. Biochem. Biophys., 1958, 74,443-450.
  • [13] Renard M., Alkhalfioui F., Schmitt-Keichinger C., RitzenthalerC., Montrichard F., Identification and characterization ofthioredoxin h isoforms differentially expressed in germinatingseeds of the model legume Medicago truncatula, PlantPhysiol., 2011, 155, 1113-1126.
  • [14] Hiroyuki Yano H., Wong J.H., Cho M.J, Buchanan, B.B., Redoxchanges accompanying the degradation of seed storageproteins in germinating rice, Plant Cell Physiol., 2001, 42,879-883.
  • [15] Dunwell J.M., Culham A., Carter C.E, Sosa-Aguirre C.R.,Goodenough P.W., Evolution of functional diversity in the cupinsuperfamily, Trends in Biochem. Sci., 2001, 26, 740-746.
  • [16] Jain, A.K., Cloning and structural analysis of a cDNA cloneencoding glycinin (Gly-1) seed storage protein of peanut, Electr.J. Biotech., 2004, 7. DOI: 10.2225/vol7-issue3-fulltext-13.[Crossref]
Document Type
Publication order reference
YADDA identifier
JavaScript is turned off in your web browser. Turn it on to take full advantage of this site, then refresh the page.