Effect of microwave power on EPR spectra of thermally sterilized eucerinum anhydricum
Languages of publication
Free radicals formed during thermal sterilization of eucerinum anhydricum – the pharmaceutical base were examined by an X-band (9.3 GHz) spectrometer. Eucerinum anhydricum was sterilized at different physical conditions according to the Polish Pharmacopeia norms. The samples were heated at temperatures: 160°C (120 min), 170°C (60 min), and 180°C (30 min). The aim of this study is to compare free radical concentration and effect of microwave power on EPR spectra of eucerinum anhydricum base thermally sterilized at different temperatures and periods of time. The effect of time storage on the free radicals in the heated samples was tested. Free radical concentrations in the sample stored 15 min strongly decreased with the increasing of sterilization temperature, probably as the result of recombination. Storage caused strong decrease of free radical concentrations in the samples, probably as the result of interactions with oxygen. It was observed to be independent of sterilization conditions from 2 days of storage and longer. Because of the lowest free radical concentration, for eucerinum anhydricum thermal sterilization at 180°C for 30 min is recommended. The sterilized samples should be stored at inert atmosphere without oxygen molecules. Fast spin-lattice relaxation processes existed in sterilized eucerinum anhydricum. The character of changes of amplitudes and linewidths of EPR lines with increasing of microwave power was the same for different storage times. The parameters of thermal sterilization and storage time influenced free radical concentration in eucerinum anhydricum, but magnetic spin-lattice interactions were unchanged. The usefulness of EPR spectroscopy in optimization of thermal sterilization process of eucerinum anhydricum was confirmed.
1 - 7 - 2015
11 - 9 - 2014
30 - 1 - 2015
6 - 8 - 2015
- 1. Polish Pharmacopoeia edition IX. (2011). Warsaw: PTFarm (in Polish).
- 2. PN-EN 556, 2005. Sterilization of medical devices. Requirement for medical devices to be as sterile. Pt. 1: Requirements for terminally sterilized medical devices; PN-EN 556, 2005. Sterilization of medical devices. Requirement for medical devices to be as sterile. Pt. 2: Requirements for medical devices manufactured under aseptic conditons. Warsaw: Polish Committee for Standardization, 2002 (in Polish).
- 3. Janicki, S., Fiebig, A., & Sznitowska, M. (2008). Farmacja stosowana. Podręcznik dla studentów farmacji, 4th ed. Warsaw: PZWL.
- 4. Jachowicz, R. (Ed.). (2008). Receptura apteczna. Podręcznik dla studentów farmacji, 2nd ed. Warsaw: PZWL.
- 5. Zaręba, M., & Borowski, J. (2013). Mikrobiologia lekarska. Warsaw: PZWL.
- 6. Krówczyński, L., & Jachowicz, R. (2000). Ćwiczenia z receptury. Krakow: Jagiellonian University.
- 7. Stankowski, J., & Hilczer, W. (2005). Wstęp do spektroskopii rezonansów magnetycznych. Warsaw: PWN.
- 8. Wertz, J. E., & Bolton, J. R. (1986). Electron spin resonance: Elementary theory and practical application. New York: Chapman and Hall.
- 9. Ramos, P., Pilawa, B., & Stroka, E. (2013). EPR studies of free radicals in thermally sterilized famotidine. Nukleonika, 58(3), 413–418.
- 10. Skowrońska, A., Wojciechowski, M., Ramos, P., Pilawa, B., & Kruk, D. (2012). ESR studies of paramagnetic centers in pharmaceutical materials – cefaclor and clarithromycin as an example. Acta Phys. Pol. A, 121(2), 514–517.
- 11. Ramos, P., Pilawa, B., & Adamski, M. (2014). Application of EPR spectroscopy for examination of thermally formed free radicals in drotaverine. Ann. Acad. Medicae Silesiensis, 68(1), 28–37 (in Polish).
- 12. Ramos, P., Pilawa, B., Krztoń, A., & Liszka, B. (2012). Free radicals in the thermally sterilized aminoglycoside antibiotics. Pharm. Anal. Acta, 3(9), 1–13.
- 13. Ramos, P., & Pilawa, B. (2009). Effect of temperature and time of thermal sterilization on formation of free radicals in isosorbide dinitrate. Farmaceutyczny Przegląd Naukowy, 7(5), 28–33 (in Polish).
- 14. Ramos, P., Pepliński, P., & Pilawa, B. (2009). Free radicals in thermally sterilized verapamil. Eng. Biomat., 12(89/91), 162–164.
- 15. Ramos, P., & Pilawa, B. (2010). The EPR examination of free radicals formation in thermally sterilized β-lactam antibiotics. Curr. Top. Biophys., 33, 183–187.
Publication order reference