Evaporative edge lithography of a liposomal drug
microarray for cell migration assays

• Authors: Nicholas Vafai , Troy W. Lowry , Korey A. Wilson , Michael W. Davidson , Steven Lenhert
• Languages of publication: EN

Abstracts

EN

Lipid multilayer microarrays are a promising
approach to miniaturize laboratory procedures by taking
advantage of the microscopic compartmentalization
capabilities of lipids. Here, we demonstrate a new method
to pattern lipid multilayers on surfaces based on solvent
evaporation along the edge where a stencil contacts a
surface called evaporative edge lithography (EEL). As an
example of an application of this process, we use EEL
to make microarrays suitable for a cell-based migration
assay. Currently existing cell migration assays require a
separate compartment for each drug which is dissolved
at a single concentration in solution. An advantage of
the lipid multilayer microarray assay is that multiple
compounds can be tested on the same surface. We
demonstrate this by testing the effect of two different
lipophilic drugs, Taxol and Brefeldin A, on collective cell
migration into an unpopulated area. This particular assay
should be scalable to test of 2000 different lipophilic
compounds or dosages on a standard microtiter plate
area, or if adapted for individual cell migration, it would
allow for high-throughput screening of more than 50,000
compounds per plate.

Keywords

EN

lithography; stencil; lipid; microarray; cellmigration; high-throughput screening

• Publisher: De Gruyter Open
• Journal: Nanofabrication
• Year: 2015
• Volume: 2
• Issue: 1

Dates

received

22 - 5 - 2015

online

24 - 7 - 2015

accepted

26 - 6 - 2015

Contributors

author

Nicholas Vafai

• Department of
  Biological Sciences, Florida State University, Tallahassee, FL, 32306-
  4370, USA

author

Troy W. Lowry

• Department of Physics, Florida State University, Tallahassee,
  FL, 32306-4350, USA

author

Korey A. Wilson

• Department of
  Biological Sciences, Florida State University, Tallahassee, FL, 32306-
  4370, USA

author

Michael W. Davidson

• National High Magnetic Field Laboratory, 1800
  East Paul Dirac Drive, Florida State University, Tallahassee, FL 32310-
  3706, USA

author

Steven Lenhert

• Department of
  Biological Sciences, Florida State University, Tallahassee, FL, 32306-
  4370, USA
• Integrative NanoScience
  Institute, Florida State University, Tallahassee, FL, 32306, USA

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Identifiers

DOI

10.1515/nanofab-2015-0004