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2015 | 2 | 1 |

Article title

Evaporative edge lithography of a liposomal drug
microarray for cell migration assays


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Lipid multilayer microarrays are a promising
approach to miniaturize laboratory procedures by taking
advantage of the microscopic compartmentalization
capabilities of lipids. Here, we demonstrate a new method
to pattern lipid multilayers on surfaces based on solvent
evaporation along the edge where a stencil contacts a
surface called evaporative edge lithography (EEL). As an
example of an application of this process, we use EEL
to make microarrays suitable for a cell-based migration
assay. Currently existing cell migration assays require a
separate compartment for each drug which is dissolved
at a single concentration in solution. An advantage of
the lipid multilayer microarray assay is that multiple
compounds can be tested on the same surface. We
demonstrate this by testing the effect of two different
lipophilic drugs, Taxol and Brefeldin A, on collective cell
migration into an unpopulated area. This particular assay
should be scalable to test of 2000 different lipophilic
compounds or dosages on a standard microtiter plate
area, or if adapted for individual cell migration, it would
allow for high-throughput screening of more than 50,000
compounds per plate.








Physical description


22 - 5 - 2015
24 - 7 - 2015
26 - 6 - 2015


  • Department of
    Biological Sciences, Florida State University, Tallahassee, FL, 32306-
    4370, USA
  • Department of Physics, Florida State University, Tallahassee,
    FL, 32306-4350, USA
  • Department of
    Biological Sciences, Florida State University, Tallahassee, FL, 32306-
    4370, USA
  • National High Magnetic Field Laboratory, 1800
    East Paul Dirac Drive, Florida State University, Tallahassee, FL 32310-
    3706, USA
  • Department of
    Biological Sciences, Florida State University, Tallahassee, FL, 32306-
    4370, USA
  • Integrative NanoScience
    Institute, Florida State University, Tallahassee, FL, 32306, USA


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