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2014 | 2 | 1 |

Article title

Transcription-Type Protein Imprinted Polymers for
SPR Sensing Prepared Using Target-immobilized
Stamps based on Submicrometer-Sized Particles
via Biotin-Avidin Linkage


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Transcribed molecularly imprinted polymers
(MIPs), prepared by using the biotinylated submicrometersized
particles-immobilized stamp on which the biotinconjugated
protein was hierarchically immobilized via
avidin, were used successfully in the recognition of
cytochrome c (Cyt) as a model protein. The transcribed MIP
for Cyt was prepared on the gold-coated surface plasmon
resonance (SPR) sensor chip, and the binding behavior of
Cyt, myoglobin, ribonuclease A, lysozyme, and avidin was
evaluated to confirm the selectivity for Cyt. The imprint
effect of the transcribed MIP was revealed by comparing
the MIP and the corresponding non-imprinted polymer
prepared using the stamp without the Cyt immobilization.







Physical description


11 - 2 - 2016
14 - 9 - 2015
24 - 11 - 2015


  • Graduate School of Engineering, Kobe University, 1-1 Rokkodai-
    cho, Nada-ku, Kobe 657-8501, Japan
  • Graduate School of Engineering, Kobe University, 1-1 Rokkodai-
    cho, Nada-ku, Kobe 657-8501, Japan
  • Graduate School of Engineering, Kobe University, 1-1 Rokkodai-
    cho, Nada-ku, Kobe 657-8501, Japan
  • Graduate School of Engineering, Kobe University, 1-1 Rokkodai-
    cho, Nada-ku, Kobe 657-8501, Japan
  • Graduate School of Engineering, Kobe University, 1-1 Rokkodai-
    cho, Nada-ku, Kobe 657-8501, Japan


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