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2014 | 2 | 1 |

Article title

Analysis of cooperative interactions in molecularly
imprinted polymer nanoparticles

Content

Title variants

Languages of publication

EN

Abstracts

EN
Cooperative binding is commonly observed
in biological receptor systems. This study investigates
whether it is possible to prepare nano-sized molecularly
imprinted polymers (nanoMIPs) that show cooperative
binding. NanoMIPs which exhibit cooperative binding
would have increased affinity for immobilised template
molecules making them useful for advanced applications
in diagnostics and sensors. The use of a templatederivatised
solid support provides a facile route to
prepare nanoMIPs with surface imprints, and the
method is ideally suited to study this topic. Although not
observed during the course of this study, positive interbinding
site cooperativity was hypothesised by way of an
increase in the number of binding sites imprinted on the
nanoMIPs, by increasing template density on the solid
support surface. After synthesis, the affinity of nanoMIPs
was analysed using surface plasmon resonance (SPR)
technique. Under the conditions investigated, a ten fold
increase in binding affinity was measured as template
density was increased. SPR results could be explained by
an increase in cooperative binding; however calculations
showed that the increase in affinity was not significant
enough to prove cooperative binding interactions. The
main conclusion obtained was that MIP nanoparticles
contain only one “high-affinity” binding site that interacts
with immobilised template in an SPR assay.

Publisher

Year

Volume

2

Issue

1

Physical description

Dates

received
30 - 6 - 2015
online
8 - 2 - 2016
accepted
9 - 9 - 2015

Contributors

author
  • Department of Chemistry, College of Science and Engineering,
    University of Leicester, LE1 7RH, UK
  • Department of Chemistry, College of Science and Engineering,
    University of Leicester, LE1 7RH, UK
author
  • Department of Chemistry, College of Science and Engineering,
    University of Leicester, LE1 7RH, UK
  • Department of Chemistry, College of Science and Engineering,
    University of Leicester, LE1 7RH, UK
author
  • Department of Chemistry, College of Science and Engineering,
    University of Leicester, LE1 7RH, UK
  • Department of Chemistry, College of Science and Engineering,
    University of Leicester, LE1 7RH, UK

References

  • [1] J. A. Goodrich and J. F. Kugel, in Binding and Kinetics forMolecular Biologists, Cold Spring Harbor Laboratory Press,New York, 2007, 49–68.
  • [2] J. E. Ferrell, Q&A: Cooperativity, J. Biol., 2009, 8, 53.[Crossref]
  • [3] M. I. Stefan and N. Le Novère, Cooperative Binding, PLoSComput. Biol., 2013, 9.
  • [4] M. Ptashne and A. Gann, Genes & Signals, Cold Spring HarborLaboratory Press, New York, 2002.
  • [5] S. A. Piletsky, N. W. Turner and P. Laitenberger, Molecularlyimprinted polymers in clinical diagnostics--future potential andexisting problems, Med. Eng. Phys., 2006, 28, 971–7.[Crossref]
  • [6] H. S. Andersson, J. G. Karlsson, S. a. Piletsky, A. C.Koch-Schmidt, K. Mosbach and I. a. Nicholls, Study of thenature of recognition in molecularly imprinted polymers, II
  • [1]: Influence of monomer-template ratio and sample load onretention and selectivity, J. Chromatogr. A, 1999, 848, 39–49.
  • [7] J. Svenson, J. G. Karlsson and I. A. Nicholls, Nuclear magneticresonance study of the molecular imprinting of (−)-nicotine:template self-association, a molecular basis for cooperativeligand binding, J. Chromatogr. A, 2004, 1024, 39–44.
  • [8] P. Sajonz, M. Kele, G. Zhong, B. Sellergren and G. Guiochon,Study of the Thermodynamics and Mass Transfer Kinetics ofTwo Enantiomers on a Polymeric Imprinted Staionary Phase, J.Chromatogr. A, 1998, 810, 1–17.
  • [9] K. Haupt, Imprinted polymers: The next generation, Anal.Chem., 2003, 75, 376–383.
  • [10] Editorial, Capturing cooperativity, Nat. Chem. Biol., 2008, 4,433.
  • [11] J. A. Goodrich and J. F. Kugel, in Binding and Kinetics forMolecular Biologists, Cold Spring Harbor Laboratory Press,New York, 2007, pp. 1 – 18.
  • [12] A. R. Guerreiro, I. Chianella, E. Piletska, M. J. Whitcombe andS. A. Piletsky, Selection of imprinted nanoparticles by affinitychromatography, Biosens. Bioelectron., 2009, 24, 2740–2743.[Crossref][WoS]
  • [13] S. A. Piletsky, K. Karim, E. V. Piletska, a. P. F. Turner, C. J.Day, K. W. Freebairn and C. Legge, Recognition of ephedrineenantiomers by molecularly imprinted polymers designedusing a computational approach, Analyst, 2001, 126,1826–1830.
  • [14] E. Moczko, A. Poma, A. Guerreiro, I. Perez de VargasSansalvador, S. Caygill, F. Canfarotta, M. J. Whitcombe and S.Piletsky, Surface-modified multifunctional MIP nanoparticles,Nanoscale, 2013, 5, 3733–41.
  • [15] Y. Hoshino, T. Kodama, Y. Okahata and K. J. Shea, Peptideimprinted polymer nanoparticles: A plastic antibody, J. Am.Chem. Soc., 2008, 130, 15242–15243.
  • [16] F. Breton, R. Rouillon, E. V. Piletska, K. Karim, A. Guerreiro, I.Chianella, S. A. Piletsky, Virtual imprinting as a tool to designefficient MIPs for photosynthesis-inhibiting herbicides,Biosens. Bioelectron., 2006, 22, 1948-1954.[WoS][Crossref]
  • [17] G. Vasapollo, R. Del Sole, L. Mergola, M. R. Lazzoi, A. Scardino,S. Scorrano and G. Mele, Molecularly imprinted polymers:present and future prospective, Int. J. Mol. Sci., 2011, 12,5908–45.[Crossref]
  • [18] J. Svenson , H. Andersson, S. A. Piletsky, I. A. Nicholls,Spectroscopic studies of the molecular imprintingself-assembly process, J. Mol. Recognit., 1998, 11, 83-86.[Crossref]
  • [19] E. V. Piletska, L. D. Stavroulakis, M. J. Whitcombe , A. Sharma,S. Primrose, G. K. Robinson, S. A. Piletsky, Passive controlof quorum sensing: prevention of pseudomonas aeruginosabiofilm formation by imprinted polymers, Biomacromolecules,2011, 12, 1066-1071.[Crossref][WoS]
  • [20] J. P. Rosengren-Holmberg, J. G. Karlsson, J. Svenson, H. S.Andersson and I. A. Nicholls, Molecularly imprinted polymers:present and future prospective, Org. Biomol. Chem., 2009, 7,3148.
  • [21] A. Poma, A. Guerreiro, M. J. Whitcombe, E. V. Piletska, A. P. F.Turner and S. a. Piletsky, Solid-Phase Synthesis of MolecularlyImprinted Polymer Nanoparticles with a Reusable Template-“Plastic Antibodies”, Adv. Funct. Mater., 2013, 23, 2821–2827.[Crossref][WoS]
  • [22] S. Subrahmanyam, A. Guerreiro, A. Poma, E. Moczko, E.Piletska and S. Piletsky, Optimisation of experimentalconditions for synthesis of high affinity MIP nanoparticles, Eur.Polym. J., 2013, 49, 100–105. [WoS][Crossref]

Document Type

Publication order reference

Identifiers

YADDA identifier

bwmeta1.element.-psjd-doi-10_1515_molim-2015-0003
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