Analysis of cooperative interactions in molecularly
imprinted polymer nanoparticles

• Authors: Jatin Mistry , Antonio Guerreiro , Ewa Moczko , Elena Piletska , Kal Karim , Sergey A. Piletsky
• Languages of publication: EN

Abstracts

EN

Cooperative binding is commonly observed
in biological receptor systems. This study investigates
whether it is possible to prepare nano-sized molecularly
imprinted polymers (nanoMIPs) that show cooperative
binding. NanoMIPs which exhibit cooperative binding
would have increased affinity for immobilised template
molecules making them useful for advanced applications
in diagnostics and sensors. The use of a templatederivatised
solid support provides a facile route to
prepare nanoMIPs with surface imprints, and the
method is ideally suited to study this topic. Although not
observed during the course of this study, positive interbinding
site cooperativity was hypothesised by way of an
increase in the number of binding sites imprinted on the
nanoMIPs, by increasing template density on the solid
support surface. After synthesis, the affinity of nanoMIPs
was analysed using surface plasmon resonance (SPR)
technique. Under the conditions investigated, a ten fold
increase in binding affinity was measured as template
density was increased. SPR results could be explained by
an increase in cooperative binding; however calculations
showed that the increase in affinity was not significant
enough to prove cooperative binding interactions. The
main conclusion obtained was that MIP nanoparticles
contain only one “high-affinity” binding site that interacts
with immobilised template in an SPR assay.

Keywords

EN

Solid-phase synthesis; template density; paracetamol; surface plasmon resonance

• Publisher: De Gruyter Open
• Journal: Molecular Imprinting
• Year: 2014
• Volume: 2
• Issue: 1

Dates

received

30 - 6 - 2015

online

8 - 2 - 2016

accepted

9 - 9 - 2015

Contributors

author

Jatin Mistry

• Department of Chemistry, College of Science and Engineering,
  University of Leicester, LE1 7RH, UK

author

Antonio Guerreiro

• Department of Chemistry, College of Science and Engineering,
  University of Leicester, LE1 7RH, UK

author

Ewa Moczko

• Department of Chemistry, College of Science and Engineering,
  University of Leicester, LE1 7RH, UK

author

Elena Piletska

• Department of Chemistry, College of Science and Engineering,
  University of Leicester, LE1 7RH, UK

author

Kal Karim

• Department of Chemistry, College of Science and Engineering,
  University of Leicester, LE1 7RH, UK

author

Sergey A. Piletsky

• Department of Chemistry, College of Science and Engineering,
  University of Leicester, LE1 7RH, UK

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Identifiers

DOI

10.1515/molim-2015-0003