Feasibility of lipid mechanical extraction
from viable Monoraphidium minutum

• Authors: Xavier Dommange , Philippe A. Tanguy , Mario Jolicoeur
• Languages of publication: EN

Abstracts

EN

Background. Mechanical stress was
investigated as a mean to harvest microalgal lipids without
affecting algal cells’ viability. Monoraphidium minutum
was cultivated in laboratory-scale photobioreactors and
suspension cultures were submitted to mechanical stress
to compare a cyclone, a centrifuge and a homogenizer.
Lipid content within the extracellular medium was
analyzed prior to and after treatment, and the amount
of released lipids was quantified. Algal cell viability
was also evaluated before and after treatment. Results.
After mechanical-stress treatments, 7.0 to 12.7% of the
intracellular lipids of Monoraphidium minutum were
released and found in the extracellular medium, while
recovered algal cells presented low levels of disruption
after treatments. Conclusions. To the best of our
knowledge, this is the first proof-of-concept demonstration
on the use of mechanical stress for lipid extraction from
viable microalgae. Certain level of centrifugation proved
to make algae release around 10% of their lipids to the
extracellular medium. This mostly exploratory work calls
for deeper investigation, paving the way for a biofuel
production based on continuous lipid recovery and
microalgae reuses.

Keywords

EN

Microalgae biofuel; lipid recovery; microalgaeviability; lipid extraction; cyclone

• Publisher: De Gruyter Open
• Journal: Microalgae Biotechnology
• Year: 2015
• Volume: 1
• Issue: 1

Dates

accepted

25 - 7 - 2014

received

27 - 12 - 2013

online

3 - 4 - 2015

Contributors

author

Xavier Dommange

• Canada Research Chair
  in Applied Metabolic Engineering. Department of Chemical
  Engineering, École Polytechnique, CP 6079 Succ. Centre-Ville,
  Montréal QC, Canada H3C 3A7
• Total SA, 2 place de la Coupole,
  La Défense 6, 92400 Courbevoie France

author

Philippe A. Tanguy

• Total SA, 2 place de la Coupole,
  La Défense 6, 92400 Courbevoie France

author

Mario Jolicoeur

• Canada Research Chair
  in Applied Metabolic Engineering. Department of Chemical
  Engineering, École Polytechnique, CP 6079 Succ. Centre-Ville,
  Montréal QC, Canada H3C 3A7

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Identifiers

DOI

10.1515/micbi-2015-0002