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2014 | 10 | 1 |

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Quick and effective method of bone marrow mesenchymal stem cell extraction


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Background. Mesenchymal stem cells (MSCs) are currently exploited in numerous clinical trials to investigate their potential in immune regulation, hematopoesis or tissue regeneration. The most common source of MSCs for clinical use is human bone marrow. To generate sufficient numbers of cells relevant to clinical use in most cases the high volumes (20-50 ml) of bone marrow aspirates are taken. Methods. In this pilot study, 8 healthy bone marrow donors were included. Two different MSC extraction methods were evaluated: MSCs extraction from 60 ml of bone marrow using density gradient and MSCs extraction from 6 ml using red blood cell (RBC) lysis. Results. Our results showed that after RBC lysis the efficient amount of human MSCs can be isolated from 10 times less bone marrow volume (6 ml). Moreover, using small volume of bone marrow the adequate therapeutical dose of MSCs could be achieved during similar period of time (3-4 weeks). In conclusion, we have shown that MSCs isolation using RBC lysis is an effective and more advantageous method in comparison to standard MSCs isolation using density-gradient. Using RBC lysis from small volume of bone marrow the same amount of MSCs were obtained as usually using large volume and density-gradient.








Physical description


1 - 1 - 2015
21 - 3 - 2014
29 - 6 - 2014
8 - 10 - 2014


  • National Cancer Institute, Biobank, Santariskiu 1, 08660 Vilnius, Lithuania, tel. +37052190909, fax. +37052720164
  • National Cancer Institute, Biobank Santariskiu 1, 08660 Vilnius, Lithuania Vilnius University, Faculty of Natural Sciences M.K. Ciurlionio 21/27, 03101 Vilnius
  • National Cancer Institute, Biobank Santariskiu 1, 08660 Vilnius, Lithuania
  • Children‘s Hospital, Affiliate of Vilnius Unversity Hospital Santariskiu Klinikos, Children Heamatology and Transplantation Center Santariskiu 7, 08406, Vilnius, Lithuania
  • Cryo-Save Labs NV, Attn. The Cell Factory (R&D department) Galileilaan 19, B-2845 Niel, Belgium


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