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Journal
2014 | 10 | 1 |
Article title

Integration of human papillomavirus type 16 in cervical cancer cells

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EN
Abstracts
EN
Cervical cancer remains an important cause of
women morbidity and mortality. The progression of cervical
pathology correlates with the HPV integration into the host
genome. However, the data on the viral integration status in
cervical dysplasias are controversial. The aim of the current
study was to evaluate the status of HPV integration in two
types of cervical pathology – invasive and non invasive
cervical cancer (e.g. carcinoma in situ). 156 women were
included in the study: 66 women were diagnosed with
invasive cervical cancer (CC) and 90 with non invasive cervical
cancer (carcinoma in situ, CIS). 74.2% [95% PI: 63.64÷84.76]
of specimens collected from women with diagnosed CC
and 85.6% [95% PI: 85.53÷92.85] of CIS specimens were
positive for HPV. The most prevalent HPV genotype in both
groups was HPV16. To evaluate HPV integration, three
selected HPV16 E2 gene fragments were analyzed by PCR.
In the majority of CC and CIS specimens the amplification
of all three HPV16 E2 gene fragments was observed. The
episomal HPV16 form was detected in the majority of CC
and CIS specimens. The deletion of all three HPV16 E2 gene
fragments was detected in 9.4% of CC specimens and 2.2%
of CIS specimens. Finally, integration status could not be
used as diagnostical additional test to distinguish between
invasive and non invasive cervical cancer.
Publisher
Journal
Year
Volume
10
Issue
1
Physical description
Dates
accepted
10 - 6 - 2014
online
25 - 9 - 2014
received
26 - 7 - 2013
References
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Document Type
Publication order reference
YADDA identifier
bwmeta1.element.-psjd-doi-10_1515_med-2015-0001
Identifiers
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