Purpose. The literature shows few studies on shod and unshod running performance in athletes, with most limited to laboratory settings. The aim of this study was to evaluate preadolescent 1000 m running performance when barefoot and in running spikes or training shoes. Methods. A sample of 22 boys and 21 girls aged 10.6 ± 1.1 years was recruited. Anthropometric data and VO2max were recorded when completing the three study protocols in a counter balanced design. Student’s t tests were applied to compare mean 1000 m finish times while ANOVA was used to evaluate sex differences between the protocols. Pearson's correlation analysis measured interactions between the finish times, anthropometric variables, and VO2max. Results. Running performance with spikes (4.58 min) was significantly better than with training shoes (5.21 min) and barefoot (5.18 min). Male 1000 m times were overall better than the females. A substantial effect of VO2max and body fat on performance was found in all protocols. Conclusions. Preadolescent endurance performance was not significantly different between training shoes and barefoot; this may serve as an incentive for future research on the training of developmental age runners.