The study investigated the accumulative effect of concentric-biased and eccentric-biased exercise on cardiorespiratory, metabolic and neuromuscular responses to low-intensity exercise performed hours later. Fourteen young men cycled at low-intensity (~60 rpm at 50% maximal oxygen uptake) for 10 min before, and 12 h after: concentric-biased, single-leg cycling exercise (CON) (performed ~19:30 h) and eccentric-biased, double-leg knee extension exercise (ECC) (~06:30 h the following morning). Respiratory measures were sampled breath-by-breath, with oxidation values derived from stoichiometry equations. Knee extensor neuromuscular function was assessed before and after CON and ECC. Cardiorespiratory responses during low-intensity cycling were unchanged by accumulative CON and ECC. The RER was lower during low-intensity exercise 12 h after CON and ECC (0.88 ± 0.08), when compared to baseline (0.92 ± 0.09; p = 0.02). Fat oxidation increased from baseline (0.24 ± 0.2 g·min1) to 12 h after CON and ECC (0.39 ± 0.2 g·min1; p = 0.01). Carbohydrate oxidation decreased from baseline (1.59 ± 0.4 g·min-1) to 12 h after CON and ECC (1.36 ± 0.4 g·min1; p = 0.03). These were accompanied by knee extensor force loss (right leg: -11.6%, p < 0.001; left leg: -10.6%, p = 0.02) and muscle soreness (right leg: 2.5 ± 0.9, p < 0.0001; left leg: 2.3 ± 1.2, p < 0.01). Subsequent concentric-biased and eccentric-biased exercise led to increased fat oxidation and decreased carbohydrate oxidation, without impairing cardiorespiration, during low-intensity cycling. An accumulation of fatiguing and damaging exercise increases fat utilisation during low intensity exercise performed as little as 12 h later.